Proteomics

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Pol IV-RDR2 protein interaction study

ABSTRACT: Protein-protein interaction between DNA dependent RNA polymerase IV and RNA dependent RNA polymerase2. The coupled activities of the physically associated polymerases lead to the biogenesis of dsRNAs that serve as precursors of 24nt siRNAs.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Yixiang Zhang  

LAB HEAD: Prof. Craig S Pikaard

PROVIDER: PXD020170 | Pride | 2020-09-14

REPOSITORIES: Pride

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Publications

Assembly of a dsRNA synthesizing complex: RNA-DEPENDENT RNA POLYMERASE 2 contacts the largest subunit of NUCLEAR RNA POLYMERASE IV.

Mishra Vibhor V   Singh Jasleen J   Wang Feng F   Zhang Yixiang Y   Fukudome Akihito A   Trinidad Jonathan C JC   Takagi Yuichiro Y   Pikaard Craig S CS  

Proceedings of the National Academy of Sciences of the United States of America 20210301 13

In plants, transcription of selfish genetic elements such as transposons and DNA viruses is suppressed by RNA-directed DNA methylation. This process is guided by 24-nt short-interfering RNAs (siRNAs) whose double-stranded precursors are synthesized by DNA-dependent NUCLEAR RNA POLYMERASE IV (Pol IV) and RNA-DEPENDENT RNA POLYMERASE 2 (RDR2). Pol IV and RDR2 coimmunoprecipitate, and their activities are tightly coupled, yet the basis for their association is unknown. Here, we show that an interva  ...[more]

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