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Redox proteomics analysis of E.coli biofilms

ABSTRACT: Proteomics analysis of planktonic and biofilm E.coli, in aerobic an anaerobic conditions. The proteomics analysis is aimed to detect the different pattern of s-nitrosylation and cysteine oxidation in the two life-styles.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Escherichia Coli

SUBMITTER: Giovanni Chiappetta

LAB HEAD: Joelle Vinh

PROVIDER: PXD020249 | Pride | 2021-05-06

REPOSITORIES: Pride

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Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress.

Barraud Nicolas N Létoffé Sylvie S Beloin Christophe C Vinh Joelle J Chiappetta Giovanni G Ghigo Jean-Marc JM

NPJ biofilms and microbiomes 20210413 1

Communities of bacteria called biofilms are characterized by reduced diffusion, steep oxygen, and redox gradients and specific properties compared to individualized planktonic bacteria. In this study, we investigated whether signaling via nitrosylation of protein cysteine thiols (S-nitrosylation), regulating a wide range of functions in eukaryotes, could also specifically occur in biofilms and contribute to bacterial adaptation to this widespread lifestyle. We used a redox proteomic approach to ...[more]

PMID: 33850153

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