Proteomics

Dataset Information

0

Three protein extraction methods for mass spectrometry proteomics of fresh frozen mouse tissue


ABSTRACT: We present three different methods for protein extraction from fresh frozen mouse heart tissue: 1) extraction using SDS buffer followed by FASP purification, 2) extraction using SDS buffer followed by S-Trap purification, and 3) extraction using a guanidine hydrochloride buffer followed by in-solution digestion. Based on bicinchoninic acid assay, all three methods display similar recovery of total protein content. However, proteomics-based analysis identified far fewer proteins from the extraction guanidine hydrochloride. SDS-FASP identifies as many proteins as the SDS-STrap method with good coverage of proteins from different cellular compartments.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Alex Kentsis  

LAB HEAD: Alex Kentsis

PROVIDER: PXD020256 | Pride | 2020-07-30

REPOSITORIES: Pride

Similar Datasets

2015-10-06 | PXD001511 | Pride
2022-08-12 | PXD031918 | Pride
2011-02-12 | GSE27225 | GEO
2018-03-08 | GSE97020 | GEO
2021-09-05 | GSE152862 | GEO
2023-02-22 | PXD039808 | Pride
2019-10-15 | GSE134101 | GEO
2019-05-21 | PXD012591 | Pride
2018-12-06 | MSV000083191 | MassIVE
2017-07-17 | PXD005969 | Pride