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Quantitative analysis of tyrosine phosphorylation from FFPE tissues reveals patient specific signaling networks

ABSTRACT: The aim of this study was to characterize tyrosine phosphorylation from FFPE tissues. Global phosphoproteomics, global proteomics and tyrosine phosphorylation were measured using quantitative LC-MS/MS based on TMT labeling.

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Lung

DISEASE(S): Glioblastoma,Breast Cancer

SUBMITTER: Ishwar Kohale

LAB HEAD: Forest Michael White

PROVIDER: PXD020284 | Pride | 2021-09-09

REPOSITORIES: Pride

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Quantitative Analysis of Tyrosine Phosphorylation from FFPE Tissues Reveals Patient-Specific Signaling Networks.

Kohale Ishwar N IN Burgenske Danielle M DM Mladek Ann C AC Bakken Katrina K KK Kuang Jenevieve J Boughey Judy C JC Wang Liewei L Carter Jodi M JM Haura Eric B EB Goetz Matthew P MP Sarkaria Jann N JN White Forest M FM

Cancer research 20210520 14

Human tissue samples commonly preserved as formalin-fixed paraffin-embedded (FFPE) tissues after diagnostic or surgical procedures in the clinic represent an invaluable source of clinical specimens for in-depth characterization of signaling networks to assess therapeutic options. Tyrosine phosphorylation (pTyr) plays a fundamental role in cellular processes and is commonly dysregulated in cancer but has not been studied to date in FFPE samples. In addition, pTyr analysis that may otherwise infor ...[more]

PMID: 34016623

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