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NanoToxClass: Investigation of dose- and time-dependent nanomaterial effects in alveolar epithelial cells

ABSTRACT: In this study, alveolar epithelial cells (cell line RLE-6TN) were treated with three different doses of nanomaterials (SiO2_15_Amino, SiO2_15_Unmod, SiO2_7, SiO2_40, TiO2_NM105) for 6 h, 24 h, and 48 h, respectively. The effects were investigated using untargeted proteomics.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Lung, Epithelial Cell

SUBMITTER: Isabel Karkossa

LAB HEAD: Kristin Schubert

PROVIDER: PXD020289 | Pride | 2021-09-29

REPOSITORIES: Pride

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Nanomaterials induce different levels of oxidative stress, depending on the used model system: Comparison of in vitro and in vivo effects.

Karkossa Isabel I Bannuscher Anne A Hellack Bryan B Wohlleben Wendel W Laloy Julie J Stan Miruna S MS Dinischiotu Anca A Wiemann Martin M Luch Andreas A Haase Andrea A von Bergen Martin M Schubert Kristin K

The Science of the total environment 20210808

The immense diversity and constant development of nanomaterials (NMs) increase the need for a facilitated risk assessment, which requires knowledge of the modes of action (MoAs) of NMs. This necessitates a comprehensive data basis, which can be obtained using omics. Furthermore, the establishment of suitable in vitro test systems is essential to follow the 3R concept and to cope with the high number of NMs. In the present study, we aimed to compare NM effects in vitro and in vivo using a multi-o ...[more]

PMID: 34428663

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