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Methodologies comparison of porcine lung tissue

ABSTRACT: Mechanistic study of H2S interference on swine, we used TMT-based comparative proteomics of lung tissues from control and hydrogen sulfide (H2S) breathing swines as a test case for evaluation of MS2, SPS MS3 and RTS-MS3 acquisition methods in both Orbitrap Fusion and Orbitrap Eclipse.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Lung, Epithelial Cell

DISEASE(S): Disease Free

SUBMITTER: Sheng Zhang

LAB HEAD: Qin Fu

PROVIDER: PXD020392 | Pride | 2023-06-02

REPOSITORIES: Pride

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Publications

Comparison of MS2, synchronous precursor selection MS3, and real-time search MS3 methodologies for lung proteomes of hydrogen sulfide treated swine.

Fu Qin Q Liu Zhen Z Bhawal Ruchika R Anderson Elizabeth T ET Sherwood Robert W RW Yang Yong Y Thannhauser Theodore T Schroyen Martine M Tang Xiangfang X Zhang Hongfu H Zhang Sheng S

Analytical and bioanalytical chemistry 20201025 2

Tandem mass tags (TMTs) have increasingly become an attractive technique for global proteomics. However, its effectiveness for multiplexed quantitation by traditional tandem mass spectrometry (MS2) suffers from ratio distortion. Synchronous precursor selection (SPS) MS3 has been widely accepted for improved quantitation accuracy, but concurrently decreased proteome coverage. Recently, a Real-Time Search algorithm has been integrated with the SPS MS3 pipeline (RTS ...[more]

PMID: 33099676

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