Proteomic analysis identifies plasma correlates of remote ischemic conditioning in the context of experimental traumatic brain injury
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Blood Plasma
SUBMITTER: 
Krystine Mansfield
LAB HEAD: Patrick Pirrotte
PROVIDER: PXD020420 | Pride | 2021-06-03
REPOSITORIES: Pride
Publications
Proteomic analysis identifies plasma correlates of remote ischemic conditioning in the context of experimental traumatic brain injury.
Saber Maha M Pathak Khyati V KV McGilvrey Marissa M Garcia-Mansfield Krystine K Harrison Jordan L JL Rowe Rachel K RK Lifshitz Jonathan J Pirrotte Patrick P
Scientific reports 20200731 1
Remote ischemic conditioning (RIC), transient restriction and recirculation of blood flow to a limb after traumatic brain injury (TBI), can modify levels of pathology-associated circulating protein. This study sought to identify TBI-induced molecular alterations in plasma and whether RIC would modulate protein and metabolite levels at 24 h after diffuse TBI. Adult male C57BL/6 mice received diffuse TBI by midline fluid percussion or were sham-injured. Mice were assigned to treatment groups 1 h a ...[more]
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Project description:Background: Remote ischaemic conditioning (RIC) is currently being explored as a non-invasive method to attenuate ischaemia/reperfusion injuries in organs. A randomised clinical study (CONTEXT) evaluated the effects of RIC compared to non-RIC controls in human kidney transplants. Methods: RIC was induced prior to kidney reperfusion by episodes of obstruction to arterial flow in the l eg opposite the transplant using a tourniquet (4 x 5min). Although RIC did not lead to clinical improvement of transplant outcomes, we explored whether RIC induced molecular changes through precision analysis of CONTEXT recipient plasma and kidney tissue samples by high-resolution tandem mass spectrometry (MS/MS). Results: We observed an accumulation of muscle derived proteins in kidney tissue proteomes and altered mitochondrial proteins, likely provoked by RIC, which was not reflected in plasma. In addition, MS/MS analysis demonstrated transient upregulation of several acute phase response proteins (SAA1, SAA2, CRP) in plasma, 1 and 5 days post-transplant. RIC showed a lower magnitude change when compared to non-RIC. However, RIC did not have a consistent effect on the magnitude of acute inflammation following clinical intervention. Conclusions: Together, our results indicate sub-clinical systemic and organ-localised effects of RIC.
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