Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic approach for searching of universal, tissue-specific and line-specific markers of extracellular vesicles in lung and colorectal adenocarcinoma cell lines: whole cell lysate vs. extracellular vesicles

ABSTRACT: The efficiency of anticancer therapy depends heavily on the molecular features of the tumor. Since tumor-derived extracellular vesicles, including exosomes, contain proteins that are characteristic for producer cells and could be detected in biological fluids, they are of great interest for screening of predictive biomarkers. We have performed label-free mass spectrometric profiling of extracellular vesicles originated from human colon cancer cell lines Caco-2, HT-26 and HCT-116, and from lung cancer cell lines NCI-H23 and A549.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer,Non-small Cell Lung Carcinoma

SUBMITTER: Svetlana Novikova

LAB HEAD: Victor Zgoda

PROVIDER: PXD020454 | Pride | 2021-09-09

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines.

Novikova Svetlana S Shushkova Natalia N Farafonova Tatiana T Tikhonova Olga O Kamyshinsky Roman R Zgoda Victor V

International journal of molecular sciences 20200909 18

Tumor-derived extracellular vesicles (EVs), including exosomes, contain proteins that mirror the molecular landscape of producer cells. Being potentially detectible in biological fluids, EVs are of great interest for the screening of cancer biomarkers. To reveal universal, tissue-specific, and line-specific markers, we performed label-free mass spectrometric profiling of EVs originating from the human colon cancer cell lines Caco-2, HT29, and HCT-116, as well as from the lung cancer cell lines N ...[more]

PMID: 32916986

Similar Datasets

Proteomic approach for searching of universal, tissue-specific and line-specific markers of extracellular vesicles in lung and colorectal adenocarcinoma cell lines:extracellular vesicles only

Project description:The efficiency of anticancer therapy depends heavily on the molecular features of the tumor. Since tumor-derived extracellular vesicles, including exosomes, contain proteins that are characteristic for producer cells and could be detected in biological fluids, they are of great interest for screening of predictive biomarkers. We have performed label-free mass spectrometric profiling of extracellular vesicles originated from human colon cancer cell lines Caco-2, HT-26 and HCT-116, and from lung cancer cell lines NCI-H23 and A549.

2021-09-09 | PXD020467 | Pride

Peptidylarginine Deiminase inhibition abolishes the production of large extracellular vesicles from Giardia intestinalis, affecting host-pathogen interactions by hindering adhesion to host cells

Project description:Giardia intestinalis is a microaerophilic protozoan that is an important etiologic agent of diarrhea worldwide. There is evidence that under diverse conditions, the parasite is capable of shedding extracellular vesicles (EVs) which modulate the physiopathology of giardiasis. Here we describe new features of G. intestinalis EV production, revealing its capacity to shed two different enriched EV populations (large (LEV) and small extracellular vesicles (SEV)) and identified relevant adhesion functions associated with the larger population. Proteomic analysis revealed differences in proteins relevant for virulence and host-pathogen interactions between the two EV subsets. We assessed the effect of two recently identified inhibitors of EV release in mammalian cells, namely peptidylarginine deiminase (PAD) inhibitor and cannabidiol (CBD), on EV release from Giardia and their putative effects on host-pathogen interactions. PAD-inhibitor Cl-amidine and CBD were both able to effectively reduce EV shedding, the PAD-inhibitor specifically affecting the release of LEVs and reducing parasite attachment to host cells in vitro. The strong efficacy of the PAD-inhibitor on Giardia EV release indicates a phylogenetically conserved pathway of PAD-mediated EV release, most likely affecting the Giardia arginine deiminase (GiADI) homolog of mammalian PADs. Our results suggest that LEVs and SEVs are differently involved in protozoa communication, and that treatment with EV-inhibitors may be a novel strategy for recurrent giardiasis treatment.

2020-09-21 | PXD018460 | Pride

Three human cell types respond to multi-walled carbon nanotubes and titanium dioxide nanobelts with cell-specific transcriptomic and proteomic expression [Caco-2/HT29-MTX cells]

Project description:To identify key biological pathways that define toxicity or biocompatibility after nanoparticle exposure, three human cell types were exposed in vitro to two high aspect ratio nanoparticles for 1 hr or 24 hr and collected for global transcriptomics. Transcriptional responses were measured by global microarray analysis of cells in culture. Groups (N=3 biological replicates) of Caco-2/HT29-MTX cells exposed to 0, 10 or 100 ug/ml MWCNT or TiO2-NB nanoparticles for 1 or 24 hr.

2012-12-15 | E-GEOD-42066 | biostudies-arrayexpress

Impaired stability of tumor cells after targeting of lactate/pyruvate metabolism investigated by shotgun proteomics

Project description:Overexpression of heat shock proteins (HSPs), especially the major stress-inducible 72 kDa heat shock protein 70 (Hsp70), in malignant tumor cells is associated with therapeutic resistance. The heightened metabolic demand in tumor cells (Warburg effect) increases lactate dehydrogenase (LDH) activity and the corresponding increase in lactate concentrations promotes malignancy. Herein, we assessed the co-regulation of LDH and the heat shock response and its link to radiation resistance in B16F10 murine melanoma cells and LS174T human colorectal adenocarcinoma cells. Inhibition of LDHA/B by oxamate or GNE-140, significantly diminishes the expression of Hsp90, Hsp70 and Hsp27 in the cytosol. Diminished expression was also observed in LDHA/B double knockout (LDH-/-) cells. An increased production of reactive oxygen species (ROS) induced by a faster growth rate in wild type (WT) vs LDH-/- cells contributes to increased cytosolic HSP levels in WT cells. LDH-/- cells exhibit a lower density of membrane Hsp70 expression than WT cells and a decreased presence of the tumor-specific, Hsp70 anchoring glycosphingolipid Gb3 on the cell surface which could be attributed to an altered lipid metabolism mediated by the LDH knockout. Functionally, a double knockout of LDHA/B results in a generalized reduction in expression of HSPs in tumor cells and significantly increases radiosensitivity which is associated with a G2/M arrest. In summary, we demonstrate that pharmacological targeting of the lactate/pyruvate metabolism breaks radioresistance by impairing the stress response.

2022-02-17 | PXD026078 | Pride

Transcription profiling of human colon Caco-2 cells treated with sulforaphane (SF)

Project description:Effect of sulforaphane (SF) on human colon caco-2 cells after 24h treatment

2005-07-01 | E-MEXP-170 | biostudies-arrayexpress

Intracellular proteins adsorbed on AuNPs during transcytosis and exocytosis

Project description:we set protein corona as the research object in this study, and focused on exploring the biological effects of intracellular protein corona (IPC) that formed on particle surface after transcytosis of nanomedicines. Considering the multi-functionalization characteristic, gold nanoparticles (AuNPs) were fabricated here as the model of nanomedicines. We established a dual-filtration proteomics strategy based on mass spectrometry (MS) to detect the composition and category of IPC after transcytosis. Based on the analysis on IPC proteins, we did achieve novel and significant findings.

2020-01-20 | PXD015651 | Pride

Exosomes from mesenchymal stromal cells reduce murine colonic inflammation via a macrophage-dependent mechanism

Project description:Conventional treatments for inflammatory bowel disease (IBD) have multiple potential side effects. Therefore, alternative treatments are desperately needed. In the present study, we proposed a new therapeutic tool for the treatment of IBD in murine pre-clinical models by using MSC-Exos. We demonstrated that the infused MSC-Exos are immunotolerated by the host, which is convenient for a future clinical application of MSC-Exos in IBD. To understand the molecular basis mediating the anticolitic benefit of MSC-Exos, we performed proteomic analysis using iTRAQ technology to detect the protein expression profiles in MSC-Exos and the corresponding supernatants from their parent cell MSCs. Proteomic analysis revealed that MSC-Exos were enriched in proteins involved in regulating multiple biological processes associated with the anti-colitic benefit of MSC-Exos. Particularly, metallothionein-2 in MSC-Exos was required for the suppression of inflammatory responses in macrophages. Taken together, MSC-Exos are critical regulators of inflammatory responses and may be promising candidates for IBD treatment.

2020-05-27 | PXD015386 | Pride

Gene expression data from 10 untreated human colon cancer cell lines

Project description:Omega-3 polyunsaturated fatty acids are normal constituents of the diet and have an essential role in maintaining important cellular functions. Docosahexaenoic acid (DHA) have demonstrated anticancer activities in several in vitro and in vivo studies, and in some clinical studies. The mechanism by which n-3 PUFAs reduce tumor growth probably involves the inhibition of cell proliferation, induction of cell death, or a combination of both. There are differences in sensitivity towards DHA treatment among colorectal cell lines, although the reason why is unclear. 10 human colorectal cell lines, representing 5 different subtypes of colorectal cancer, were included in gene expression analysis. (Pleae refer to Sadanandam A, Lyssiotis CA, Homicsko K, Collisson EA, Gibb WJ, Wullschleger S, Ostos LC, Lannon WA, Grotzinger C, Del Rio M, et al. “A colorectal cancer classification system that associates cellular phenotype and responses to therapy”. Nat Med. 2013;19:619-625. doi:10.1038/nm.3175 for details on cancer subtype classifications.) Our aim is to identify different levels of genes and pathways correlating with differences in sensitivity towards DHA between the colorectal cell lines for future classification of patients that could benefit from omega-3 PUFA treatment in addition to conventional treatment.

2018-05-08 | E-MTAB-5750 | biostudies-arrayexpress

Transcription profiling by array of human Caco-2 cells with ATP8B1 knock down

Project description:To gain insights whether and if activation of the nuclear receptor FXR is involved in the disease ethiology of ATP8B1 deficiency, we contstructed a Caco-2 ATP8B1 knock down cell line and a Caco-2 empty vector control cell line. Furthermore we expect to gain new insights from transcriptional profiling of ATP8B1 deficient cells.

2010-12-31 | E-TABM-989 | biostudies-arrayexpress

Proteomic analysis of extracellular vesicles derived from Merkel cell carcinoma cell lines

Project description:Exosomes constitute an evolutionary conserved mechanism of intercellular signaling. Their importance are gaining increasing such as prognostic and diagnostic markers, and potential therapeutic tool. Merkel cell carcinoma (MCC) is an aggressive form of skin cancer with a poor prognosis. There are not available an effective systemic treatment for this type of cancer, and exosome-based therapy was proposed. We identified with high confident 164 exosome-derived proteins that were common for all four cell lines, which were annotated in ExoCarta and Vesiclepedia databases. This list includes proteins implicated in motility, metastasis and tumor progression.

2016-08-08 | PXD004198 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data