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Proteomics of Human Blood and Semen Exosomes: Set 2

ABSTRACT: Our analyses uncover enrichment of exosome-based protein signatures specific to blood and semen along with identification of tissue-dependent and -independent perturbations in blood and semen exosomes.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Semen, Blood

DISEASE(S): Acquired Immunodeficiency Syndrome

SUBMITTER: Siddharth Pratap

LAB HEAD: Siddharth Pratap, PhD

PROVIDER: PXD020487 | Pride | 2022-06-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	All_proteins_12-20-2019.csv	Csv
	CVDash-Okeoma_02-2019_HumEx_HIVCoc_MPIT_s10_step-10.zip	Other
	CVDash-Okeoma_02-2019_HumEx_HIVCoc_MPIT_s11_step-10.zip	Other
	CVDash-Okeoma_02-2019_HumEx_HIVCoc_MPIT_s12_step-10.zip	Other
	CVDash-Okeoma_02-2019_HumEx_HIVCoc_MPIT_s13_step-10.zip	Other

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Publications

HIV-infection and cocaine use regulate semen extracellular vesicles proteome and miRNAome in a manner that mediates strategic monocyte haptotaxis governed by miR-128 network.

Kaddour Hussein H Kopcho Steven S Lyu Yuan Y Shouman Nadia N Paromov Victor V Pratap Siddharth S Dash Chandravanu C Kim Eun-Young EY Martinson Jeremy J McKay Heather H Epeldegui Marta M Margolick Joseph B JB Stapleton Jack T JT Okeoma Chioma M CM

Cellular and molecular life sciences : CMLS 20211222 1

<h4>Background</h4>Extracellular vesicles (EVs) are regulators of cell-cell interactions and mediators of horizontal transfer of bioactive molecules between cells. EV-mediated cell-cell interactions play roles in physiological and pathophysiological processes, which maybe modulated by exposure to pathogens and cocaine use. However, the effect of pathogens and cocaine use on EV composition and function are not fully understood.<h4>Results</h4>Here, we used systems biology and multi-omics analysis ...[more]

PMID: 34936021

Publication: 1/2

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Proteomics of Human Blood and Semen Exosomes

Project description:Our analyses uncover enrichment of exosome-based protein signatures specific to blood and semen along with identification of tissue-dependent and -independent perturbations in blood and semen exosomes.

2019-11-06 | PXD013033 | Pride

Evaluation of the effectiveness of semen collection and sperm purification methods for spermatozoa transcript profiling

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Proteomic analysis of fresh, equilibrated, and frozen/thawed turkey semen in relation to the extracellular fluid proteome

Project description:The cryopreservation of avian semen without significant loss of fertilizing ability is of practical interest to the poultry industry and for the conservation of genetic biodiversity. However, the unique physiological features make avian spermatozoa highly sensitive to damage caused by cryopreservation. The proteomic changes in turkey (Meleagris gallopavo) semen throughout the cryopreservation process have never been investigated previously. Therefore, the purpose of the present study was to evaluate the proteomic changes of extracellular fluid (ECF)of semen during cryopreservation at three stages (fresh, equilibrated, and frozen/thawed semen) using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF/TOF). Six protein spots were significantly enriched in ECF proteome after equilibration compared to fresh semen. Twenty seven protein spots were enriched in ECF after cryopreservation compared to fresh semen. Twenty six protein spots were significantly enriched in ECF proteome after cryopreservation compared to equilibrated semen. The proteins enriched in ECF after cryopreservation were mainly related to NADH metabolic and glycolytic process.

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Bovine semen

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Proteomic analysis of fresh, equilibrated, and frozen/thawed turkey semen in relation to spermatozoa proteome

Project description:The cryopreservation of avian semen without significant loss of fertilizing ability is of practical interest to the poultry industry and for the conservation of genetic biodiversity. However, the unique physiological features make avian spermatozoa highly sensitive to damage caused by cryopreservation. The potential proteomic changes in turkey (Meleagris gallopavo) semen throughout the cryopreservation process have never been investigated previously. Therefore, the purpose of the present study was to evaluate the proteomic changes of spermatozoa during cryopreservation at three stages (fresh, equilibrated, and frozen/thawed semen) using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF/TOF). No differentially expressed proteins (DEPs) were found in spermatozoa proteome of fresh and equilibrated semen. One hundred forty six protein spots differed significantly between fresh and frozen/thawed spermatozoa. One hundred three protein spots differed significantly between equilibrated and frozen/thawed spermatozoa. Most of the proteins that changed in response to cryopreservation were related to the generation of precursor metabolites and energy through aerobic respiration, flagellum-dependent cell motility, and binding of sperm to the zona pellucida. This suggests the complexity of the processes leading to decreases in the quality of cryopreserved semen.

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Bull semen

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Proteomic analysis of rabbit fresh and cryopreserved semen (seminal plasma)

Project description:Cryoinjury and protein changes are a consequence of cryopreservation and may have a negative impact on sperm quality regarding motility, viability and fertilizing ability. However, potential proteomic changes in rabbit semen throughout the cryopreservation process have never been investigated previously. The aim of the present study was to compare the whole proteome of fresh and cryopreserved rabbit extracellular fluid of semen. Comparative analysis and identification of proteins were performed using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass (MALDI TOF/TOF) spectrometry. We found that 28 proteins differed in abundance between fresh and frozen extracellular fluid.

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Viral fallout: Semen proteome reveals COVID-19 dysregulates several biological processes linked to male reproductive function

Project description:The current global outbreak of COVID-19 has significantly impacted several organ systems. Male reproductive organs are among the potential targets of SARS-CoV-2 infection owing to the presence of abundant viral receptors; ACE2 and TMPRSS2 in the testis. However, the questions pertaining to the long term effects of SARS-CoV-2 infection on male fertility are still unanswered. For this study, we procured the semen samples of COVID-19 recovered patients and performed mass spectrometry based proteomic studies to understand the impact of the disease on the biological processes involved in reproductive health.

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Proteomic characterization of fresh spermatozoa and supernatant after cryopreservation in relation to freezability of carp (Cyprinus carpio) semen

Project description:Our recent studies suggested that the freezability of carp semen is related to seminal plasma protein profiles. Here, we aimed to compare the spermatozoa proteomes of good (GF) and poor (PF) freezability semen of carp. To achieve this, we used two-dimensional difference in gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. The semen was classified as GF or PF based on sperm motility after freeze/thawing. We identified proteins enriched in spermatozoa of GF (22 proteins) and PF (18 proteins) semen. We also identified 12 proteins enriched in the supernatant after cryopreservation of PF semen. Good freezability is related to high concentrations of proteins involved in the maintenance of flagella structure, membrane fluidity, efficient control of Ca2+ and sperm motility, energy production, and antioxidative protection, which likely reflects the full maturation status of spermatozoa of GF semen. On the other hand poor freezability seems to be related to the presence of proteins identified as released in high quantities from cryopreserved sperm of PF. Thus, the identified proteins might be useful bioindicators of freezing resilience and could be used to screen carp males before cryopreservation, thus improve long-term sperm preservation in carp.

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