PASEF technology on timsTOF Pro improves quality of FPOP analysis

ABSTRACT: Fast photochemical oxidation of proteins (FPOP) is a modern technique for studying protein folding, conformations, interactions, etc. In this work, timsTOF Pro mass spectrometer coupled with trapped ion mobility spectrometry was used for identification of oxidative modifications in a model protein. Multiple modifications on the same residues, including six modifications of histidine, were successfully resolved. Moreover, PASEF technology allows successful sequencing even minor populations of modified peptides.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Dmitry Loginov  

LAB HEAD: Petr Novak

PROVIDER: PXD020509 | Pride | 2021-06-10

REPOSITORIES: Pride