Identification of bacteriolytic lysosomal proteins in Dictyostelium discoideum by LC-MSMS

ABSTRACT: In order to identify new D. discoideum proteins involved in the intracellular destruction of ingested bacteria, the amoeba cell lysate was fractionated, and the obtained fractions were screened for their bacteriolytic activity. The few fractions presenting a bacteriolytic activity were analyzed by mass spectrometry in order to identify the microbicidal effectors.

INSTRUMENT(S):

ORGANISM(S): Dictyostelium Discoideum (slime Mold)

TISSUE(S): Cell Culture

SUBMITTER: Cyril Guilhen  

LAB HEAD: Pierre Cosson

PROVIDER: PXD020632 | Pride | 2021-01-20

REPOSITORIES: Pride