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Host-dependent NCR profiles of Rhizobium leguminosarum bacteroids induced in pea and lentil plants.

ABSTRACT: We have determined the profile of plant-derived NCR peptides present in endosymbiotic cells (bacteroids) induced by R. leguminosarum bv viciae strain UPM791 in nodules from two different hosts (pea and lentil).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rhizobium Leguminosarum Bv. Viciae

SUBMITTER: Jose Palacios

LAB HEAD: Jose Palacios

PROVIDER: PXD020634 | Pride | 2020-11-27

REPOSITORIES: Pride

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Proteome Analysis Reveals a Significant Host-Specific Response in Rhizobium leguminosarum bv. viciae Endosymbiotic Cells.

Durán David D Albareda Marta M García Carlos C Marina Ana-Isabel AI Ruiz-Argüeso Tomás T Palacios Jose-Manuel JM

Molecular & cellular proteomics : MCP 20201206

The Rhizobium-legume symbiosis is a beneficial interaction in which the bacterium converts atmospheric nitrogen into ammonia and delivers it to the plant in exchange for carbon compounds. This symbiosis implies the adaptation of bacteria to live inside host plant cells. In this work, we apply RP-LC-MS/MS and isobaric tags as relative and absolute quantitation techniques to study the proteomic profile of endosymbiotic cells (bacteroids) induced by Rhizobium leguminosarum bv viciae strain UPM791 i ...[more]

PMID: 33214187

Similar Datasets

Proteome analysis reveals a significant host-specific response in Rhizobium leguminosarum bv viciae endosymbiotic cells

Project description:We study the proteomic profile of endosymbiotic cells (bacteroids) induced by R. leguminosarum bv viciae strain UPM791 in nodules from two different hosts. Comparative analysis of bacteroids induced in pea and lentil nodules revealed the existence of a significant host-specific differential response affecting multiple bacterial proteins, and also revealed the presence of different sets of plant-derived nodule-specific cysteine rich (NCR) peptides.

2020-11-27 | PXD020631 | Pride

Transcription profiling of bacteroid Rhizobium leguminosarum strain 3841 wild type vs bacA mutant harvested from pea plant nodules

Project description:Wildtype bacteroids of Rhizobium leguminosarum biovar viciae 3841 were compared to a bacA mutant. In both cases bacteroids were harvested from 7 day old nodules from pea plants.

2009-01-06 | E-MEXP-1966 | biostudies-arrayexpress

Transcription profiling of Rhizobium leguminosarum biovar viciae strain 3841 inoculated onto pea seeds compared to cells grown in free-living culture on succinate ammonia AMS

Project description:Rhizobium leguminosarum bv viciae strain 3841 was inoculated onto pea (Pisum sativum) seeds and nodules were harvested at 28 d. The gene expression was compared to free-living bacteria grown on succinate ammonia AMS medium.

2008-11-30 | E-MEXP-1925 | biostudies-arrayexpress

Proteomic screening for potentially amyloidogenic proteins of Rhizobium leguminosarum in effective and ineffective root nodules of Pisum sativum L.

Project description:We studied potentially amyloidogenic proteins (e.g. protein forming polymers and complexes that are resistant to treatment with ionic detergents) in root nodules formed by two lines of garden pea (P. sativum L.): Sprint-2 (Fix+ phenotype) and Sprint-2Fix- (sym31) (Fix- phenotype) inoculated with the Rhizobium leguminosarum bv. viciae RCAM1026 root nodule bacteria. The Fix+ phenotype is characterized by effective (ability to fix nitrogen) root nodules formation. The Fix- line is a descendant of the Fix+ line and forms ineffective root nodules (unable to fix nitrogen) with undifferentiated bacteroids. We demonstrated the presence of both plant and bacterial proteins in detergent resistant fractions, including previously identified amyloid proteins RopA and RopB of R. leguminosarum and vicilin of P. sativum L.

2022-10-25 | PXD035724 | Pride

Transcription profiling by array of Rhizobium leguminosarum biovar viciae strain Rlv3841 grown rhizosphere of either pea, alfalfa or sugarbeet

Project description:Rhizobium leguminosarum biovar viciae strain Rlv3841 was grown for 7 days in the rhizosphere of either 7 day old pea, alfalfa or sugarbeet before being harvested.

2010-08-03 | E-MEXP-2849 | biostudies-arrayexpress

Succinate transport is not essential for symbiotic nitrogen fixation by Sinorhizobium meliloti nor Rhizobium leguminosarum

Project description:Symbiotic nitrogen fixation (SNF) is an energetically expensive process performed by bacteria known as rhizobia during endosymbiotic relationships with leguminous plants. The bacteria require the plant to provide a carbon source for generation of the reductant to power SNF. Although it is well known that C4-dicarboxylates (succinate, fumarate, malate) function as the primary, if not sole, carbon source provided to the rhizobia, the relative contribution of each C4-dicarboxylate is not known. Here, we employ genetic and systems-level analyses to address this issue. Expression of a malate specific transporter (MaeP) in Sinorhizobium meliloti Rm1021 dct mutants unable to transport C4-dicarboxylates resulted in malate import rates up to ~ 30% that of wild type S. meliloti. This was sufficient to support SNF with Medicago sativa, with acetylene reduction rates up to ~ 50% those of plants inoculated with wild type S. meliloti. Rhizobium leguminosarum bv. viciae 3841 dct mutants unable to transport C4-dicarboxylates but expressing the maeP transporter had strong symbiotic properties, with Pisum sativum plants inoculated with these strains appearing similar to plants inoculated with wild type R. leguminosarum. This was despite malate transport rates by the mutant bacteroids being < 10% those of the wild type. A transcriptomics analysis of the combined plant/bacterium nodule transcriptome was performed using RNA-sequencing to identify any systems-level adaptations in response to the inability of the bacteria to import succinate or fumarate. Few transcriptional changes, with no obvious pattern, were revealed by this analysis. Overall, these data illustrated that succinate and fumarate are not essential for SNF, and that at least in specific symbioses, L-malate is likely to naturally serve as the primary C4-dicarboxylate provided to the bacterium.

2017-09-16 | GSE101430 | GEO

Transcription profiling by array of Rhizobium leguminosarum bivar viciae strain Rlv3841 grown on pyruvate ammonia AMS with the addition of pea root washing

Project description:Rhizobium leguminosarum bivar viciae strain Rlv3841 was grown in the laboratory on pyruvate (10mM) ammonia (10mM) AMS. The effect of addition of pea root washing was compared to an untreated control.

2010-10-02 | E-MEXP-2846 | biostudies-arrayexpress

Transcription profiling of Rhizobium leguminosarum biovar viciae strain 3841 inoculated onto vetch seeds compared to cells grown in free-living culture on succinate ammonia AMS

Project description:Rhizobium leguminosarum biovar viciae strain 3841 was inoculated onto Viccia cracca seeds and bacteroids were harvested at 28d. Gene expression was then compared to free-living Rhizobium leguminosarum biovar viciae strain 3841 grown on succinate ammonia AMS.

2008-11-30 | E-MEXP-1924 | biostudies-arrayexpress

Transcription profiling by array of Rhizobium leguminosarum Rlv 3841 from pea plants infected for 7, 14 or 21 days compared to bacteria grown on glucose amonia

Project description:Pea plants of 7, 14 or 21 were infected with Rhizobium leguminosarum biovar viciae strain Rlv3841 and bacterial gene expression measured 24 hours later. Expression was compared to bacteria grown in the laboratory on glucose ammonia.

2010-10-01 | E-MEXP-2845 | biostudies-arrayexpress

Transcription profiling by array of Rhizobium leguminosarum biovar viciae strain Rlv3841grown in pea, alfalfa or sugarbeet rhizospheres compared to bacteria grown on glucose ammonia AMS medium

Project description:Rhizobium leguminosarum biovar viciae strain Rlv3841 was grown for 7 d in the pea or alfalfa or sugarbeet rhizosphere (plants all 7 d old at inoculation) and compared against bacteria grown on glucose ammonia AMS medium in the laboratory.

2010-08-04 | E-MEXP-2852 | biostudies-arrayexpress

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