Improved sensitivity of ultra-low flow LC-MS-based proteomic profiling of limited samples using monolithic capillary columns and FAIMS technology

ABSTRACT: In this work, we pioneered a combination of ultra-low flow (ULF) high-efficiency ultra-narrow bore monolithic LC columns coupled to MS via a high-field asymmetric waveform ion mobility spectrometry (FAIMS) interface to evaluate the potential applicability for high sensitivity, robust and reproducible proteomic profiling of low ng-level complex biological samples.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Michal Gregus  

LAB HEAD: Alexander R. Ivanov

PROVIDER: PXD020669 | Pride | 2021-01-10

REPOSITORIES: Pride