Proteomics

Dataset Information

0

Neurospora crassa eisosome LC-MS


ABSTRACT: To identify proteins that localize to MCC/eisosomes in N. crassa, we isolated proteins that co-purified with the core MCC/eisosome protein LSP-1, which was tagged with GFP. Proteins that co-fractionated with LSP-1::GFP were then identified by mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Neurospora Crassa Or74a

SUBMITTER: Qin Yang  

LAB HEAD: Frank Kempken

PROVIDER: PXD020724 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

The Composition and the Structure of MCC/Eisosomes in <i>Neurospora crassa</i>.

Yang Qin Q   Kempken Frank F  

Frontiers in microbiology 20200918


MCC/eisosomes are protein-organized domains in the plasma membrane of fungi and algae. However, the composition and function(s) of MCC/eisosomes in the filamentous fungus <i>Neurospora crassa</i> were previously unknown. To identify proteins that localize to MCC/eisosomes in <i>N. crassa</i>, we isolated proteins that co-purified with the core MCC/eisosome protein LSP-1, which was tagged with GFP. Proteins that co-fractionated with LSP-1:GFP were then identified by mass spectrometry. Eighteen pr  ...[more]

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