Quantitative LC-MS/MS Proteomics of human skin and LabSkin model

ABSTRACT: We report for the first time label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in six human skin explants and a 3D living skin equivalent model from LabSkin. More than 2000 proteins were identified and quantified from total cellular protein. This systematic determination of functional equivalence between human skin and LabSkin is a key step towards the construction of a representative human in vitro skin model, which can be used as an alternative to current animal-based tests for chemical safety and for predicting dosage of topically administered drugs.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin

SUBMITTER: Jill Barber  

LAB HEAD: Jill Barber

PROVIDER: PXD020742 | Pride | 2021-09-08

REPOSITORIES: Pride