Affinity purification of biotinylated AtACINUS and Mass Spectrometric analysis to identify PTMs on AtACINUS

ABSTRACT: AtACINUS protein is involved in regulation of alternative transcription and splicing (AS). Previously we have identified O-GlcNAcylation and phosphorylation sites on AtACINUS using LWAC and IMAC enrichment (Xu et al., 2017). To identify more modification sites, we have created AtACINUS-YFP-TurboID/acinus-2pinin transgenic lines and pulled down AtACINUS using streptavidin beads to identify post-translational modifications on AtACINUS.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Shoot

SUBMITTER: Shouling Xu  

LAB HEAD: SHOULING XU

PROVIDER: PXD020749 | Pride | 2020-12-02

REPOSITORIES: Pride