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Uncovering the pathways related with extracellular vesicles derived from Devil Facial Tumour cells (DFTD).

ABSTRACT: Tasmanian devils are threatened with extinction because of two genetically independent transmissible cancers which are termed as Devil Facial Tumour Disease one and two (DFTD-1 and DFTD-2). The cancers are indistinguishable by gross examination and require laboratory procedures such as immunohistochemistry and/or PCR for diagnosis. Here we used proteomics to analyse extracellular vesicles from DFTD cells to better understand the potentail role of EV's in DFTD cancer metastasis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sarcophilus Harrisii

TISSUE(S): Cell Culture

SUBMITTER: Richard Wilson

LAB HEAD: Dr Richard Wilson

PROVIDER: PXD020766 | Pride | 2023-04-27

REPOSITORIES: Pride

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Extracellular vesicle proteomes of two transmissible cancers of Tasmanian devils reveal tenascin-C as a serum-based differential diagnostic biomarker.

Espejo Camila C Wilson Richard R Willms Eduard E Ruiz-Aravena Manuel M Pye Ruth J RJ Jones Menna E ME Hill Andrew F AF Woods Gregory M GM Lyons A Bruce AB

Cellular and molecular life sciences : CMLS 20211016 23

The iconic Tasmanian devil (Sarcophilus harrisii) is endangered due to the transmissible cancer Devil Facial Tumour Disease (DFTD), of which there are two genetically independent subtypes (DFT1 and DFT2). While DFT1 and DFT2 can be differentially diagnosed using tumour biopsies, there is an urgent need to develop less-invasive biomarkers that can detect DFTD and distinguish between subtypes. Extracellular vesicles (EVs), the nano-sized membrane-enclosed vesicles present in most biofluids, repres ...[more]

PMID: 34655299

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