An integrated multi-omics protocol for swine feces reveals congruent longitudinal dynamics in microbiome structure and function.

ABSTRACT: Today, swine is regarded as promising biomedical model, however, its gastrointestinal microbiome dynamics have been less investigated than that of humans or murine models . The aim of this study was to establish a high-throughput multi-omics pipeline to investigate the healthy fecal microbiome of swine and its temporal dynamics as basis for future infection studies. To this end, a homogenization protocol based on deep-frozen feces followed by integrated sample preparation for different meta-omics analyses was developed. Subsequent data integration linked microbiome composition with function, i.e. expressed proteins and secreted metabolites.

INSTRUMENT(S):

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

SUBMITTER: Daniela Zuehlke  

LAB HEAD: Katharina Riedel

PROVIDER: PXD020775 | Pride | 2021-02-15

REPOSITORIES: Pride