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A comprehensive spectral assay library to quantify the Escherichia coli proteome by DIA/SWATH-MS

ABSTRACT: A comprehensive and high-quality E. coli spectral assay library for 56,182 proteotypic peptides mapped to 4,014 (91.5%) of the 4,389 annotated proteins using one- and two-dimensional fractionated samples, and ion mobility separation.

INSTRUMENT(S): TripleTOF 5600, TripleTOF 6600

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Mukul Midha

LAB HEAD: Prof. Robert L. Moritiz

PROVIDER: PXD020785 | Pride | 2020-10-19

REPOSITORIES: Pride

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A comprehensive spectral assay library to quantify the Escherichia coli proteome by DIA/SWATH-MS.

Midha Mukul K MK Kusebauch Ulrike U Shteynberg David D Kapil Charu C Bader Samuel L SL Reddy Panga Jaipal PJ Campbell David S DS Baliga Nitin S NS Moritz Robert L RL

Scientific data 20201112 1

Data-Independent Acquisition (DIA) is a method to improve consistent identification and precise quantitation of peptides and proteins by mass spectrometry (MS). The targeted data analysis strategy in DIA relies on spectral assay libraries that are generally derived from a priori measurements of peptides for each species. Although Escherichia coli (E. coli) is among the best studied model organisms, so far there is no spectral assay library for the bacterium publicly available. Here, we generated ...[more]

PMID: 33184295

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