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PhosTip for Enrichment of Phosphopeptides from Urinary Extracellular Vesicles

ABSTRACT: Extracellular vesicles (EVs) are attracting increasing interest with their intrigue role in intercellular communications. Protein phosphorylation in EVs is of great importance for understanding intercellular signaling processes. However, the study of EV phosphoproteomics is impeded by their relatively low amount in limited clinical samples and it is necessary to have a sensitive and efficient enrichment method for EV phosphopeptides. Herein, a novel Ti4+-functionalized and glass-fiber supported hybrid monolithic spin tip, we named PhosTip, was prepared for enriching phosphopeptides from urinary EVs.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

SUBMITTER: Haiyang Zhang

LAB HEAD: W. Andy Tao

PROVIDER: PXD020861 | Pride | 2022-02-15

REPOSITORIES: Pride

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Glass Fiber-Supported Hybrid Monolithic Spin Tip for Enrichment of Phosphopeptides from Urinary Extracellular Vesicles.

Zhang Haiyang H Deng Yuanyuan Y Liu Xinyi X Sun Jie J Ma Leyao L Ding Yajie Y Zhan Zhen Z Zhang Hao H Yang Yuchen Y Gu Yanhong Y Iliuk Anton B AB Yang Chenxi C Tao W Andy WA

Analytical chemistry 20201019 21

Extracellular vesicles (EVs) are attracting increasing interest with their intriguing role in intercellular communications. Protein phosphorylation in EVs is of great importance for understanding intercellular signaling processes. However, the study of EV phosphoproteomics is impeded by their relatively low amount in limited clinical sample volumes, and it is necessary to have a sensitive and efficient enrichment method for EV phosphopeptides. Herein, a novel Ti(IV)-functionalized and glass fibe ...[more]

PMID: 33074658

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