Protein binding to PDCD4 promoter X-box in Nutlin-3a treated U2OS cells

ABSTRACT: Human PDCD4 wild-type (wt) promoter fragments were amplified from U2OS genomic DNA and X-box deletion mutants (mut) were generated using the QuikChange site-directed mutagenesis kit (Agilent Technologies). DNA probes for affinity purification with the same sequences were obtained by PCR using a biotinylated primer for labeling the 3' end (Thermo Fisher Scientific). DNA affinity purification with nuclear extracts from Nutlin-3a treated U2OS cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Alessandro Ori  

LAB HEAD: Martin Fisher

PROVIDER: PXD020932 | Pride | 2024-08-08

REPOSITORIES: Pride