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EC-exosomes and BM fluid-Exosomes

ABSTRACT: We isolated the exosomes from mouse bone marrow fluid, mouse primary bone marrow endothelial cells (BMEC) culture supernatants of WT-BMEC and Vps33b knockdown BMECs by ultracentrifugation. After characterization of isolated exosomes, A global comparison of the protein cargo carried by the different exosomes. In brief，protein were extracted and labeled with TMT, the peptide were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in a Q Exactive HF-X (Thermo) coupled with an EASY-nLC 1200 UPLC system, and the resulting MS/MS data were processed using the MaxQuant search engine.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Guohuan Sun

LAB HEAD: Guohuan Sun

PROVIDER: PXD020946 | Pride | 2020-11-24

REPOSITORIES: Pride

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ANGPTL2-containing small extracellular vesicles from vascular endothelial cells accelerate leukemia progression.

Huang Dan D Sun Guohuan G Hao Xiaoxin X He Xiaoxiao X Zheng Zhaofeng Z Chen Chiqi C Yu Zhuo Z Xie Li L Ma Shihui S Liu Ligen L Zhou Bo O BO Cheng Hui H Zheng Junke J Cheng Tao T

The Journal of clinical investigation 20210101 1

Small extracellular vesicles (SEVs) are functional messengers of certain cellular niches that permit noncontact cell communications. Whether niche-specific SEVs fulfill this role in cancer is unclear. Here, we used 7 cell type-specific mouse Cre lines to conditionally knock out Vps33b in Cdh5+ or Tie2+ endothelial cells (ECs), Lepr+ BM perivascular cells, Osx+ osteoprogenitor cells, Pf4+ megakaryocytes, and Tcf21+ spleen stromal cells. We then examined the effects of reduced SEV secretion on pro ...[more]

PMID: 33108353

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