Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Propionate production from carbon monoxide by synthetic co-cultures of Acetobacterium wieringae spp. and propionigenic bacteria

ABSTRACT: This study was conducted in order to evaluate the physiological ability for syngas conversion into propionate by a synthetic co-culture. A proteogenomics approach was used to get insight into the metabolic pathways of the conversion of CO to propionate.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Anaerotignum Neopropionicum Dsm 3847 Acetobacterium Wieringae

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD020960 | Pride | 2021-05-10

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DMS10_Well_3Slice2.raw	Raw
	DMS11_Well_3Slice3.raw	Raw
	DMS12_Well_3Slice4.raw	Raw
	DMS13_Well_4Slice1.raw	Raw
	DMS14_Well_4Slice2.raw	Raw

Items per page:

1 - 5 of 37

Publications

Propionate Production from Carbon Monoxide by Synthetic Cocultures of Acetobacterium wieringae and Propionigenic Bacteria.

Moreira João P C JPC Diender Martijn M Arantes Ana L AL Boeren Sjef S Stams Alfons J M AJM Alves M Madalena MM Alves Joana I JI Sousa Diana Z DZ

Applied and environmental microbiology 20210625 14

Gas fermentation is a promising way to convert CO-rich gases to chemicals. We studied the use of synthetic cocultures composed of carboxydotrophic and propionigenic bacteria to convert CO to propionate. So far, isolated carboxydotrophs cannot directly ferment CO to propionate, and therefore, this cocultivation approach was investigated. Four distinct synthetic cocultures were constructed, consisting of Acetobacterium wieringae (DSM 1911<sup>T</sup>) and Pelobacter propionicus (DSM 2379<sup>T</su ...[more]

PMID: 33990298

Similar Datasets

Investigation on the anaerobic propionate degradation by Escherichia coli K12

Project description:Propionate is an abundant carboxylic acid in nature. Microorganisms metabolize propionate aerobically via the 2-methylcitrate pathway. This pathway depends on a series of three reactions in the citric acid cycle that leads to the conversion of succinate to oxaloacetate. Interestingly, the gamma-proteobacterium Escherichia coli can use propionate as a carbon and electron source under oxic but not under anoxic conditions. The typical downregulation of the citric acid cycle under anoxic conditions is only partially responsible for the inability to use propionate under anoxic conditions since an arcA mutant shows very limited growth on propionate. RT-PCR and transcriptomic analysis revealed a post-transcriptional regulation of the prp-genecluster encoding the necessary enzymes for propionate metabolism. The polycistronic mRNA was hydrolyzed in the 3`-5` direction under anoxic conditions. This regulatory strategy is highly constructive because the last gene of the operon encodes the first enzyme of the propionate metabolism. Further analysis revealed that RNase R catalyzes the hydrolysis of the prp transcripts. Consequently, an rnr-deletion strain could metabolize propionate under anoxic conditions. To the best of our knowledge, this is the first study describing the influence of RNase R on the anaerobic metabolism of E. coli.

2017-12-14 | GSE78814 | GEO

Propionate converting anaerobic microbial communities enriched from distinct biogeochemical zones of Aarhus Bay, Denmark under sulfidogenic and methanogenic conditions

Project description:Propionate conversion in Aarhus Bay enrichments

| PRJEB36640 | ENA

bulk RNA sequencing for GL261 cells treated with propionate

Project description:To identify the effect of propionate on GL261 cells, we treated GL261 cells with sodium chloride or propionate and extracted RNA at 24 hours post-treatment

2025-04-23 | GSE233412 | GEO

Propionate axis in infancy small intestinal eosinophils

Project description:Evidence has accumulated that gut microbiota and its metabolites, in particular, a short chain fatty acid propionate, are significant contributors to the pathogenesis of a variety of diseases, although little is known regarding its impact on pediatric bronchial asthma, one of the most common allergic diseases in childhood. This study aimed to elucidate whether, and if so how, intestinal propionate during lactation is involved in the development of BA. In order to perform a bias-free and comprehensive genetic screening of intestinal eosinophils, we next performed RNA sequencing analysis on SILP eosinophils sorted from offspring mice of mothers fed with propionate-containing water or control water

2022-07-11 | GSE207711 | GEO

Altered propionate metabolism contributes to tumor progression and aggressiveness

Project description:The alteration of metabolic pathways is a critical strategy for cancer cells to attain the traits necessary for metastasis in disease progression. We found that dysregulation of propionate metabolism occurs through TGFbeta- and ERK2-driven downregulation of methylmalonyl-CoA epimerase (MCEE). MCEE downregulation occurs through a transcription factor Sp1/EGR1 switch at its promoter region, resulting in reduced flux through this anapleurotic pathway and leading to accumulation of methylmalonic acid (MMA), a byproduct of the propionate metabolism. MMA accumulation through alteration of propionate metabolism produces a pro-aggressive signature in breast and lung cancer cells and increases their metastatic potential. Altogether, we present a previously uncharacterized dysregulation of propionate metabolism as a novel contributor to cancer and a valuable potential target in the therapeutic treatment of metastatic carcinomas.

2022-01-28 | GSE161108 | GEO

Bulk RNA sequencing for GL261 cells treated with propionate (4h post-treatment)

Project description:To identify the effect of propionate on GL261 cells, we treated GL261 cells with sodium chloride or propionate and extracted RNA at 4 hours post-treatment

2025-04-23 | GSE260955 | GEO

Meg-01 cell line expression data after propionate stimulation

Project description:Platelets are produced by megakaryocytes, deriving from megakaryocyte erythrocyte progenitors (MEP) in the bone marrow. Increased megakaryocyte expansion across common autoimmune diseases was shown for rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and primary Sjögren’s syndrome (pSS). In this context, we evaluated the role of the microbial-derived short chain fatty acid (SCFA) propionate on megakayriocytes in vitro. Using RNA sequencing we characterized the consequences of propionate exposure on the megakaryoblast cell line Meg-01.

2022-05-14 | GSE202712 | GEO

Disrupted propionate metabolism evokes transcriptional changes in the heart by increasing histone acetylation and propionylation [ChIP-Seq 1]

Project description:Propiogenic substrate catabolism and gut bacteria produce propionate, a post-translational protein modifier. Here, using a mouse model of propionic acidaemia (PA), we characterise how disturbances to propionate metabolism modify histones and affect cardiac gene expression and function. Plasma propionate was raised in PA mice, but male hearts accumulated a smaller propionyl-CoA excess, correlating with -alanine build-up. Raising -alanine experimentally in myocytes cultured under propionate-stress reduced propionyl-CoA and partially reversed the propionate-evoked metabolic disturbance. Female PA hearts manifested a moderate diastolic dysfunction phenotype, with raised diastolic [Ca2+], expanded end-systolic ventricular volume, and reduced stroke volume. Differentially-expressed genes included Pde9a and Mme that relate to contractile dysfunction through down-scaled cGMP signalling, consistent with phosphoproteome changes. Propionate was traced to histone H3 propionylation and increased acetylation genome-wide, including at Pde9a and Mme promoters, with more pronounced effects in female PA hearts. We link perturbed propionate metabolism to epigenetic changes that impact cardiac function.

2023-09-28 | GSE205836 | GEO

Disrupted propionate metabolism evokes transcriptional changes in the heart by increasing histone acetylation and propionylation [RNA-Seq 2]

2023-09-28 | GSE205835 | GEO

Disrupted propionate metabolism evokes transcriptional changes in the heart by increasing histone acetylation and propionylation [RNA-Seq 1]

2023-09-28 | GSE205834 | GEO