Murine alveolar macrophage cell line MH-S was transduced with lentivirus expressing GFP alone or GFP plus murine MFSD7C tagged with Myc and FLAG epitopes.

ABSTRACT: We used immunoprecipitation (IP) and mass spectrometry (MS) to identify proteins that interact with MFSD7C. Because an anti-MFSD7C monoclonal antibody is not available, we used MFSD7C tagged with both Myc and FLAG epitopes in a MFSD7C-expressing murine alveolar macrophage cell line MH-S (Supplementary Fig. 2a-c and see Methods for details). A total of 58 proteins were identified excluding MFSD7C and common contaminants of IP experiments such as keratin, actin, and ribosomal proteins (Table 1). Twenty-six of the 58 proteins are annotated as mitochondrial proteins and 4 (three Fc receptors and one integrin) are known to reside in the cytoplasmic membrane. The 58 proteins were classified into 10 different functional categories based on gene ontology.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Proliferative Vasculopathy And Hydranencephaly-hydrocephaly Syndrome

SUBMITTER: Yingzhong Li  

LAB HEAD: Jianzhu Chen

PROVIDER: PXD021016 | Pride | 2020-08-20

REPOSITORIES: Pride