Proteomics

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The Ubiquitin-modified Proteome of SARS-CoV-2-infected Host Cells Reveals Insights into Virus-Host Interaction and Pathogenesis


ABSTRACT: The outbreak of coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has posed a serious threat to global public health. The mechanism of pathogenesis and host immune response to SARS-CoV-2 infection are largely unknown. In the present study, we applied a quantitative proteomic technology to identify and quantify the ubiquitination changes that occur in both the virus and Vero cells during SARS-CoV-2 infection. By applying label-free, quantitative LC-MS/MS proteomics, 8,943 lysine ubiquitination sites on 3,086 proteins were identified, of which 138 sites on 104 proteins were quantified as significantly upregulated, while 828 sites on 447 proteins were downregulated at 72 h post-infection. Bioinformatics analysis suggested that SARS-CoV-2 infection might modulate host immune responses through the ubiquitination of important proteins, including USP5, IQGAP1, TRIM28, and Hsp90. Ubiquitination modification was also observed on 11 SAR-CoV-2 proteins, including proteins involved in virus replication and inhibition of the host innate immune response. Our study provides new insights into the interaction between SARS-CoV-2 and the host as well as potential targets for the prevention and treatment of COVID-19.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sars Coronavirus Gd322

TISSUE(S): Cell Culture

SUBMITTER: Jinying Zhu  

LAB HEAD: Zeliang Chen

PROVIDER: PXD021097 | Pride | 2021-03-08

REPOSITORIES: Pride

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Publications

Ubiquitin-Modified Proteome of SARS-CoV-2-Infected Host Cells Reveals Insights into Virus-Host Interaction and Pathogenesis.

Zhang Huan H   Zheng Huanying H   Zhu Jinying J   Dong Qiao Q   Wang Jin J   Fan Huahao H   Chen Yangzhen Y   Zhang Xi X   Han Xiaohu X   Li Qianlin Q   Lu Jiahai J   Tong Yigang Y   Chen Zeliang Z  

Journal of proteome research 20210305 5


The outbreak of coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has posed a serious threat to global public health. The mechanism of pathogenesis and the host immune response to SARS-CoV-2 infection are largely unknown. In the present study, we applied a quantitative proteomic technology to identify and quantify the ubiquitination changes that occur in both the virus and the Vero E6 cells during SARS-CoV-2 infection. By applyi  ...[more]

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