Dataset Information

Title- Modulation of implanted aortic scaffold- involvement of ECM and immune proteins

ABSTRACT: Development of a suitable biological scaffold is an essential pre-requisite for functional development of organ including blood vessel. Biological scaffold prepared by conventional means using physical or chemical agent have been shown to cause damage to the extracellular matrix architecture as well as produce immune response subsequent to scaffold implantation/engraftment. During the current study, taking advantage of different host tissue environment (subcutaneous vs omentum), acellular scaffold was prepared using a syngeneic vs allogeneic model of scaffold implantation (in vivo decellularization) and compared with a chemically decellularized scaffold. By means of molecular, proteomics and histologic techniques, we confirmed that site specific advantages exist in modulating the ECM as well as regulating the immune response (macrophage and T-cells) to produce an acellular scaffold without the need for immunosuppressants. The current approach opens up the possibility for creation of tailor made scaffold for further use in the clinics with a possibility for long term acceptability without adverse reactions in the host and a further possibility to build functional organs following cell engraftment.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat) Rattus Rattus (black Rat)

TISSUE(S): Blood Vessel

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Carina Sihlbom

LAB HEAD: Carina Sihlbom

PROVIDER: PXD021179 | Pride | 2026-07-06

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2982_SP-rat_11plex-MS2quant_QEHF_181005.msf	Msf
	2982_SP-rat_11plex-MS2quant_QEHF_181005_1_FDRproteins_quant-in-all-channels.xlsx	Xlsx
	QEHF_181005_06.raw	Raw
	QEHF_181005_07.raw	Raw
	QEHF_181005_08.raw	Raw

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Publications

Characterization of Decellularized Implants for Extracellular Matrix Integrity and Immune Response Elicitation.

Banerjee Debashish D Nayakawde Nikhil B NB Antony Deepti D Deshmukh Meghshree M Ghosh Sudip S Sihlbom Carina C Berger Evelin E Ul Haq Uzair U Olausson Michael M

Tissue engineering. Part A 20220621 13-14

Biological scaffold is a popular choice for the preparation of tissue-engineered organs and has the potential to address donor shortages in clinics. However, biological scaffolds prepared by physical or chemical agents cause damage to the extracellular matrix (ECM) by potentially inducing immune responses after implantation. The current study explores the fate of the decellularized (DC) scaffolds using a cocktail of chemicals following implantation without using immunosuppressants. Using the syn ...[more]

PMID: 34963315

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