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Structure of TFIIK for phosphorylation of CTD of RNA polymerase II

ABSTRACT: TFIIH is a 10-protein complex that is conserved through out eukaryotes. TFIIH has two primary cellular functions: transcription initiation and nucleotide excision repair (NER). In transcription initiation, TFIIH acts as a structural scaffold, phosphorylates the RNA polymerase II (pol II) C-terminal domain (CTD) and translocates promoter DNA through the pol II active site to facilitate start site selection. In NER, again is a structural scaffold, opens a bubble around damaged DNA and scans the damaged strand for bulky lesions. In yeast (Saccharomyces cerevisiae), TFIIH is composed of the two helicases Ssl2 and Rad3, the scaffolding subunits Tfb1, Tfb2, Tfb4 and Ssl1 and the kinase subunits Kin28, Ccl1 and Tfb3.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Hee Jong Kim

LAB HEAD: Benjamin Aaron Garcia

PROVIDER: PXD021211 | Pride | 2021-07-07

REPOSITORIES: Pride

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Structure of TFIIK for phosphorylation of CTD of RNA polymerase II.

van Eeuwen Trevor T Li Tao T Kim Hee Jong HJ Gorbea Colón Jose J JJ Parker Mitchell I MI Dunbrack Roland L RL Garcia Benjamin A BA Tsai Kuang-Lei KL Murakami Kenji K

Science advances 20210407 15

During transcription initiation, the general transcription factor TFIIH marks RNA polymerase II by phosphorylating Ser5 of the carboxyl-terminal domain (CTD) of Rpb1, which is followed by extensive modifications coupled to transcription elongation, mRNA processing, and histone dynamics. We have determined a 3.5-Å resolution cryo-electron microscopy (cryo-EM) structure of the TFIIH kinase module (TFIIK in yeast), which is composed of Kin28, Ccl1, and Tfb3, yeast homologs of CDK7, cyclin H, and MA ...[more]

PMID: 33827808

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