Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Fasnall induces atpically transient Stress Granules

ABSTRACT: Stress Granule formation has been linked to the resistance of some cancer cells to chemotherapeutic intervention. A number of studies have proposed that certain anti-tumor compounds promote cancer cell survival by inducing Stress Granules formation, leading to increased cellular fitness and apoptosis avoidance. Here we show that a potent fatty acid synthase inhibitor, fasnall, known for its anti-tumor capabilities, triggers the formation of non-canonical Stress Granules with a faster clearing and internal dynamics kinetics independently of fatty acid synthase inhibition. PABPC1 BioID interactomes in fasnall, and arsenite conditions were obtained.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Triana Amen

LAB HEAD: Daniel Kaganovich

PROVIDER: PXD021229 | Pride | 2021-09-09

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Fasnall Induces Atypically Transient Stress Granules Independently of FASN Inhibition.

Amen Triana T Kaganovich Daniel D

iScience 20200910 10

Stress Granule formation has been linked to the resistance of some cancer cells to chemotherapeutic intervention. A number of studies have proposed that certain anti-tumor compounds promote cancer cell survival by inducing Stress Granule formation, leading to increased cellular fitness and apoptosis avoidance. Here we show that a potent fatty acid synthase inhibitor, fasnall, known for its anti-tumor capabilities, triggers the formation of atypical Stress Granules, independently of fatty acid sy ...[more]

PMID: 33083719

Similar Datasets

PABPC1 stress granule interactome identified by BioID

Project description:Identification of Stress Granule interacting proteins by BioID (Roux et al., 2012) using PABPC1-BirA(R118G)-GFP construct

2022-02-17 | PXD025334 | Pride

Identification of Stress Granule interactor proteins in yeast S. cerevisiae

Project description:We investigated the sub-cellular architecture of Stress Granules in the context of glucose starvation. In order to uncover transient and peripheral Stress Granule interactors, we optimized the proximity-dependent biotinylation (BioID) approach for use in yeast. This approach allowed us to visualize Stress Granule formation while simultaneously inducing the biotinylation of SG components and nearby proteins in live cells. We used two independent Stress Granule components Pub1 and Pab1.

2020-03-23 | PXD016988 | Pride

HeberleRazquinNavas2019 - The PI3K and MAPK/p38 pathways control stress granuleassembly in a hierarchical manner model 3

Project description:All cells and organisms exhibit stress-coping mechanisms toensure survival. Cytoplasmic protein-RNA assemblies termedstress granules are increasingly recognized to promote cellularsurvival under stress. Thus, they might represent tumor vul-nerabilities that are currently poorly explored. The translation-inhibitory eIF2αkinases are established as main drivers ofstress granule assembly. Using a systems approach, we identifythe translation enhancers PI3K and MAPK/p38 as pro-stress-granule-kinases. They act through the metabolic master regu-lator mammalian target of rapamycin complex 1 (mTORC1) topromote stress granule assembly. When highly active, PI3K is themain driver of stress granules; however, the impact of p38becomes apparent as PI3K activity declines. PI3K and p38 thusact in a hierarchical manner to drive mTORC1 activity and stressgranule assembly. Of note, this signaling hierarchy is also presentin human breast cancer tissue. Importantly, only the recognition ofthe PI3K-p38 hierarchy under stress enabled the discovery of p38’srole in stress granule formation. In summary, we assign a new pro-survival function to the key oncogenic kinases PI3K and p38, as theyhierarchically promote stress granule formation

2020-01-03 | BIOMD0000000907 | BioModels

HeberleRazquinNavas2019 - The PI3K and MAPK/p38 pathways control stress granuleassembly in a hierarchical manner model 2

Project description:All cells and organisms exhibit stress-coping mechanisms to ensure survival. Cytoplasmic protein-RNA assemblies termed stress granules are increasingly recognized to promote cellular survival under stress. Thus, they might represent tumor vulnerabilities that are currently poorly explored. The translation-inhibitory eIF2α kinases are established as main drivers of stress granule assembly. Using a systems approach, we identify the translation enhancers PI3K and MAPK/p38 as pro-stress-granule-kinases. They act through the metabolic master regulator mammalian target of rapamycin complex 1 (mTORC1) to promote stress granule assembly. When highly active, PI3K is the main driver of stress granules; however, the impact of p38 becomes apparent as PI3K activity declines. PI3K and p38 thus act in a hierarchical manner to drive mTORC1 activity and stress granule assembly. Of note, this signaling hierarchy is also present in human breast cancer tissue. Importantly, only the recognition of the PI3K-p38 hierarchy under stress enabled the discovery of p38’s role in stress granule formation. In summary, we assign a new pro-survival function to the key oncogenic kinases PI3K and p38, as they hierarchically promote stress granule formation.

2020-01-03 | MODEL2001030002 | BioModels

ECT2 and AURKB Modulate Formation of Stress Granules Containing Transcripts from Diverse Cellular Pathways in Astrocytoma Cells

Project description:Stress granules are small RNA-protein granules that modify the translational landscape during cellular stress to promote survival. The RhoGTPase RhoA is implicated in the formation of RNA stress granules. Our data demonstrate that the cytokinetic proteins ECT2 and AurkB are localized to stress granules in human astrocytoma cells. AurkB and its downstream target histone-3 are phosphorylated during arsenite-induced stress. Chemical (AZD1152-HQPA) and siRNA inhibition of AurkB results in fewer and smaller stress granules when analyzed utilizing high throughput fluorescent based cellomics assays. RNA immunoprecipitation with the known stress granule aggregates TIAR and G3BP1 was performed on astrocytoma cells and subsequent analysis revealed that astrocytoma stress granules harbour unique mRNAs for various cellular pathways including cellular migration, metabolism, translation and transcriptional regulation. Human astrocytoma cell stress granules contain mRNA that are known to be involved in glioma signaling and the mTOR pathway. These data provide evidence that RNA stress granules are a novel form of epigenetic regulation in astrocytoma cells, which may be targetable by chemical inhibitors and enhance astrocytoma susceptiblity to conventional therapy such as radiation and chemotherapy. Astrocytoma cells were either untreated or treated with arsenite to induce stress granule formation and RNA immunoprecipitates were analyzed by exon array analysis. RNA species that were enriched in TIAR RIPs and G3BP1 RIPS, respectively were compared to compared to TIAR and G3BP1 RIPs from untreated cells and input controls. Ingenuity pathway analysis was performed on the stress granule enriched mRNAs from the TIAR and G3BP1 RIPs to identify significant functional biology networks.

2016-02-29 | E-GEOD-76814 | biostudies-arrayexpress

Ribosome profiling on heat shocked wild type and Mettl14 -/- mES cells

Project description:Abstract : "A hallmark of the cellular response to environmental stress is the formation of stress granules. Stress granules are RNA-protein assemblies that provide an adaptive response to stress; however, the basis for their formation and how they contribute to the stress response remains incompletely understood. Here we show that the mRNA modification N6-methyladenosine (m6A) is a mark that targets mRNAs to stress granules. We find that m6A mRNAs are highly enriched in stress granules, and this is mediated by m6A-induced liquid-liquid phase separation of the YTHDF family of m6A-binding proteins. These proteins bind poly-methylated m6A mRNAs, causing them to form liquid droplets that partition into stress granules. Moreover, disrupting either m6A or YTHDF proteins prevents stress granule formation." The goal of this experiment is to understand how recruitment of m6A mRNA to stress granules influences the translational response to heat shock. Result: we found that m6A-containing mRNA are preferentially repressed during stress, and that m6A is required for translational recovery after heat shock

2021-01-15 | GSE109253 | GEO

Protein and metabolite composition of the Arabidopsis stress granules.

Project description:Stress granules are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Herein, we report protein composition of the Arabidopsis SGs supporting their role in plant response to stress. Moreover, and to our knowledge for the first time, we provide evidence for not only mRNAs and proteins, but also phospholipids and amino-acids accumulation in the SGs. A quarter of the identified proteins constituted known or predicted SGs components, supporting evolutionary conserved mechanism of SGs assembly and dynamics. Intriguingly, remaining proteins were enriched in key enzymes and regulators mediating plant response to stress, such as cyclin dependent kinase A (CDKA), pointing to the important role of SGs in moderating plant response to stress by selective storage and temporary inactivation of the proteins. We hypothesize that phospholipid sequestration into SGs may serve partly analogous role providing protection from the phospholipase mediated degradation occurring upon combination of heat and dark stress.

2022-03-27 | PXD010455 | Pride

ECT2 and AURKB Modulate Formation of Stress Granules Containing Transcripts from Diverse Cellular Pathways in Astrocytoma Cells

2016-02-29 | GSE76814 | GEO

Tudor Staphylococcal Nuclease (TSN) proteome isolation

Project description:Stress granules (SGs) are transient membraneless organelles protecting yeast and mammalian cells from stress-induced damage, whereas plant SGs remain poorly understood. Recently, we identified Tudor Staphylococcal Nuclease (TSN) as a stable component of Arabidopsis SGs. As a first step to investigate the role of TSN in stress granule formation, we characterised its proteome by using an alternative tandem affinity purification (TAPa).

2021-06-11 | PXD018095 | JPOST Repository

The stress granule transcriptome reveals principles of mRNA accumulation in stress granules.

Project description:Stress granules are mRNA-protein assemblies formed on nontranslating mRNAs. Stress granules are important in the stress response, related to neuronal mRNP granules, and aberrant stress granules contribute to some degenerative diseases. By RNA-Seq and single molecule FISH, we describe the stress granule transcriptome in both yeast and mammalian cells. This reveals that while essentially every mRNA, and some ncRNAs, can be targeted to stress granules, the efficiency of targeting can vary from <1% to 73%. mRNA accumulation in stress granules is increased by longer coding regions, poor translatability, and correlates with some RNA binding proteins. Standardizing the RNA-Seq analysis by single molecule FISH allows a quantitative description of the general and stress granule transcriptome. Approximately 15% of the bulk mRNA molecules accumulate in stress granules suggesting their effect will be limited primarily to subsets of mRNAs highly accumulating in stress granules

2017-11-10 | GSE99304 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data