Proteomics

Dataset Information

0

Comparative analysis of PAD4 and SAG101 interactors upon activation of NLR immunity in Arabidopsis thaliana

ABSTRACT: Intracellular NLR receptors with a central nucleotide-binding domain allow plants to detect specific pathogen-secreted proteins and initiate immune signaling. A family of conserved Enhanced disease susceptibility 1 proteins with a lipase-like and the EP domain transduces the signal from activated NLRs downstream. This family includes EDS1, PAD4 and SAG101 that form mutually exclusive heterodimers EDS1-PAD4 and EDS1-SAG101. Despite sequence similarities, PAD4 and SAG101 control different aspects of NLR immune signaling. More specifically, PAD4 and SAG101 regulate resistance and cell death outputs of the NLR receptor pair RRS1-RPS4, respectively. Also, PAD4 and SAG101 genetically cooperate with distinct downstream signaling NLRs - ADR1 and NRG1. The main aim of this IP-LC/MS project is to assess whether PAD4 and SAG101 form complexes with ADR1 and NRG1 and to gain insight into mechanisms of the genetically established functional links between EDS1 family proteins and downstream signaling NLRs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Sara Christina Stolze  

LAB HEAD: Hirofumi Nakagami

PROVIDER: PXD021301 | Pride | 2021-04-06

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Plants utilise intracellular nucleotide-binding, leucine-rich repeat (NLR) immune receptors to detect pathogen effectors and activate local and systemic defence. NRG1 and ADR1 "helper" NLRs (RNLs) cooperate with enhanced disease susceptibility 1 (EDS1), senescence-associated gene 101 (SAG101) and phytoalexin-deficient 4 (PAD4) lipase-like proteins to mediate signalling from TIR domain NLR receptors (TNLs). The mechanism of RNL/EDS1 family protein cooperation is not understood. Here, we present g  ...[more]

Similar Datasets

Proteomics The EDS1-PAD4-ADR1 node mediates Arabidopsis pattern-triggered immunity
2021-07-05 | PXD026120 | Pride
Proteomics Cavity surface residues of PAD4 and SAG101 contribute to EDS1 dimer signaling specificity in plant immunity
2022-03-17 | PXD029477 | Pride
Transcriptomics Time-course RNA-seq analysis of the barley MLA1 immune receptor-mediated response to barley powdery mildew fungus Bgh in Arabidopsis thaliana
2012-11-22 | E-GEOD-39463 | biostudies-arrayexpress
Proteomics Proteomic analysis of FPA interactors
2020-12-16 | PXD022684 | Pride
Proteomics Isolation of Arabidopsis EDS1 complexes from leaves with activated TNL RPS4/RRS1 signaling
2021-04-06 | PXD025202 | Pride
Unknown RRM Transcription Factors Interact with NLRs and Regulate Broad-Spectrum Blast Resistance in Rice
2019-08-04 | GSE118356 | GEO
Unknown RRM Transcription Factors Interact with NLRs and Regulate Broad-Spectrum Blast Resistance in Rice
2019-08-04 | GSE118355 | GEO
Unknown RRM Transcription Factors Interact with NLRs and Regulate Broad-Spectrum Blast Resistance in Rice
2019-08-04 | GSE118352 | GEO
Unknown Study of primary transcriptional changes induced by EDS1/PAD4 in Arabidopsis using an estradiol-inducible system
2016-09-27 | GSE80585 | GEO
Proteomics Data in support of Identification of potential autoantigens in anti-CCP-positive and anti-CCP-negative rheumatoid arthritis
2022-01-05 | PXD024955 | Pride