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PI3Kalpha Immunocomplex Identification

ABSTRACT: Mass Spectrometry Analysis of PI3Ka Immunocomplex via immunoprecipitation and SDS-PAGE separation

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Greg Sabat

LAB HEAD: Dr. Richard A. Anderson

PROVIDER: PXD021306 | Pride | 2022-02-16

REPOSITORIES: Pride

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Phosphatidylinositol-3-OH kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4.

Thapa Narendra N Chen Mo M Horn Hudson T HT Choi Suyong S Wen Tianmu T Anderson Richard A RA

Nature cell biology 20201102 11

The canonical model of agonist-stimulated phosphatidylinositol-3-OH kinase (PI3K)-Akt signalling proposes that PI3K is activated at the plasma membrane, where receptors are activated and phosphatidylinositol-4,5-bisphosphate is concentrated. Here we show that phosphatidylinositol-3,4,5-trisphosphate generation and activated Akt are instead largely confined to intracellular membranes upon receptor tyrosine kinase activation. Microtubule-associated protein 4 (MAP4) interacts with and controls loca ...[more]

PMID: 33139939

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