Proteomics

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Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation.

ABSTRACT: Protein composition of human ovarian follicular fluid (FF) constitutes the microenvironment for oocyte development. Several proteomics studies of FF from pre-ovulatory follicles have revealed insights on oocyte maturation, however, there is a lack of knowledge on changes produced at protein levels in the FF of human small antral follicles (hSAF) related to the upcoming oocyte maturation. Using mass spectrometry-based proteomics, we evaluated the protein composition of FF that surrounds oocytes capable to reach metaphase II (MII) after IVM with the protein profile of FF that surrounds immature oocytes. The samples were collected from small antral follicles (size 6.0 ± 1.5 mm) extracted from six women, from which two or three samples were extracted. The comparison was based on both, a multivariate (sPLS-DA) and univariate analyses (t-test).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Follicular Fluid

DISEASE(S): Female Reproductive System

SUBMITTER: Indira Pla Parada  

LAB HEAD: Johan Malm

PROVIDER: PXD021338 | Pride | 2021-09-09

REPOSITORIES: Pride

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Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation.

Pla Indira I   Sanchez Aniel A   Pors Susanne Elisabeth SE   Pawlowski Krzysztof K   Appelqvist Roger R   Sahlin K Barbara KB   Poulsen Liv La Cour LC   Marko-Varga György G   Andersen Claus Yding CY   Malm Johan J  

Human reproduction (Oxford, England) 20210201 3

<h4>Study question</h4>Is it possible to identify by mass spectrometry a wider range of proteins and key proteins involved in folliculogenesis and oocyte growth and development by studying follicular fluid (FF) from human small antral follicles (hSAF)?<h4>Summary answer</h4>The largest number of proteins currently reported in human FF was identified in this study analysing hSAF where several proteins showed a strong relationship with follicular developmental processes.<h4>What is known already</  ...[more]

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