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Analysis of Gcn20 pullout samples by mass spectrometry

ABSTRACT: Mass spectrometric analysis of Gcn20 pullout samples.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Sergo Kasvandik

LAB HEAD: Prof. Dr. Daniel Wilson

PROVIDER: PXD021365 | Pride | 2021-09-09

REPOSITORIES: Pride

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Structure of Gcn1 bound to stalled and colliding 80S ribosomes.

Pochopien Agnieszka A AA Beckert Bertrand B Kasvandik Sergo S Berninghausen Otto O Beckmann Roland R Tenson Tanel T Wilson Daniel N DN

Proceedings of the National Academy of Sciences of the United States of America 20210401 14

The Gcn pathway is conserved in all eukaryotes, including mammals such as humans, where it is a crucial part of the integrated stress response (ISR). Gcn1 serves as an essential effector protein for the kinase Gcn2, which in turn is activated by stalled ribosomes, leading to phosphorylation of eIF2 and a subsequent global repression of translation. The fine-tuning of this adaptive response is performed by the Rbg2/Gir2 complex, a negative regulator of Gcn2. Despite the wealth of available bioche ...[more]

PMID: 33790014

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