Proteomics

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The temperature quickly modulates protein expression by Codon Usage in wheat filling grain


ABSTRACT: The protein content determines the cell state. The variation in protein abundance is crucial when organisms are in the early stages of heat stress, but the reasons affecting their changes are largely unknown. We quantified 47,535 mRNAs and 3,742 proteins in filling grain of wheat under two thermal environments. The impact of mRNA abundance and sequence features which implicated in protein translation and degradation on protein expression was evaluated by regression analysis. Transcription, codon usage and amino acid frequency mainly drive the changes in protein expression under heat stress, and their combined contribution explains 58.2% and 66.4% of protein variation in 30 and 40 °C, respectively. Of which, transcription contributes more to the alteration in protein content under 40 °C (31%) than to 30 °C (6%). Codon usage plays a stable and powerful role in protein expression under heat stress, even surpassing transcription. What’s more, the usage of AAG is a key factor regulating rapid protein expression under heat stress.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Plant Cell, Kernel

SUBMITTER: Bingjin Wu  

LAB HEAD: Bingjin Wu

PROVIDER: PXD021460 | Pride | 2021-03-24

REPOSITORIES: Pride

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