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Identifying the BACE1 interactome through BioID proximity assay

ABSTRACT: This study used the BioID proximity assay to establish the BACE1 interactome in healthy neuronal cells and identified interactions involved in BACE1 trafficking, post-translational modification and substrates.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hippocampal Cell Line

SUBMITTER: Michele Tinti

LAB HEAD: Michael L.J. Ashford

PROVIDER: PXD021464 | Pride | 2021-12-30

REPOSITORIES: Pride

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Identifying the beta-site amyloid precursor protein cleaving enzyme 1 interactome through the proximity-dependent biotin identification assay.

Gabriel Jennie L JL Tinti Michele M Fuller William W Ashford Michael L J MLJ

Neuroscience letters 20211025

Beta-site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1) is a key drug target against Alzheimer's Disease however, due to its promiscuous proteolytic activity, little is known about its physiological functions. Previous studies have analysed BACE1 cleavage products to examine BACE1 interactions and determine substrates, but these studies cannot establish non-enzymatic (and potentially functional) associations. This study used the biotin identification proximity assay to establish the ...[more]

PMID: 34710551

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