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An engineered promoter-reporter for insoluble protein in Saccharomyces cerevisiae

ABSTRACT: Saccharomyces cerevisiae proteome response upon expression of 3 proteins (mKate2, mKate2-MBP and mKate2-LipPks1) was studied by shotgun proteomics.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: David Romero-Suarez

LAB HEAD: Michael Krogh Jensen

PROVIDER: PXD021470 | Pride | 2021-02-22

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	PD_Contaminants_2015_5.fasta	Fasta
	Prot36.mgf	Mgf
	Prot36.msf	Msf
	Prot36.mzIdentML	Mzid
	Prot36.pep.xml	Pepxml

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Publications

A Reporter System for Cytosolic Protein Aggregates in Yeast.

Romero-Suarez David D Wulff Tune T Rong Yixin Y Jakočiu Nas Tadas T Yuzawa Satoshi S Keasling Jay D JD Jensen Michael K MK

ACS synthetic biology 20210212 3

Protein misfolding and aggregation are linked to neurodegenerative diseases of mammals and suboptimal protein expression within biotechnology. Tools for monitoring protein aggregates are therefore useful for studying disease-related aggregation and for improving soluble protein expression in heterologous hosts for biotechnology purposes. In this work, we developed a promoter-reporter system for aggregated protein on the basis of the yeast native response to misfolded protein. To this end, we fir ...[more]

PMID: 33577304

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