Dataset Information

Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: a Proteomics study

ABSTRACT: This work aims to study the main metabolic pathways in lettuce tissues contaminated with three different pharmaceuticals: metformin MTF, carbamazepine CBZ and acetaminophen ACT . The main focus of this proteomic analysis will be the relation among stress related proteins and specific metabolic pathways regarding defence mechanisms. In order to accomplish this, previous results obtained from the direct study of enzymatic activities will be correlated with the results obtained from this proteomic analysis. This work will then contribute to improve knowledge regarding the contamination of lettuce with three largely used pharmaceutical compounds.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Lactuca Sativa

TISSUE(S): Plant Cell, Root, Leaf

DISEASE(S): Pharmaceutical

SUBMITTER: Céline LECLERCQ

LAB HEAD: Céline LECLERCQ

PROVIDER: PXD021474 | Pride | 2021-06-09

REPOSITORIES: Pride

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Publications

Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study.

Leitão Inês I Leclercq Céline C CC Ribeiro David M DM Renaut Jenny J Almeida André M AM Martins Luisa L LL Mourato Miguel P MP

Journal of proteomics 20210604

Pharmaceutical compounds have been found in rivers and treated wastewaters. They often contaminate irrigation waters and consequently accumulate in edible vegetables, causing changes in plants metabolism. The main objective of this work is to understand how lettuce plants cope with the contamination from three selected pharmaceuticals using a label free proteomic analysis. A lettuce hydroponic culture, grown for 36 days, was exposed to metformin, acetaminophen and carbamazepine (at 1 mg/L), duri ...[more]

PMID: 34089899

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Project description:BACKGOUND: Drinking water can be contaminated with pharmaceuticals. However, it is uncertain whether this contamination can have harmful consequences for the liver, especially in the context of obesity. OBJECTIVES: To determine whether chronic, low dose exposure to pharmaceuticals could have deleterious effects in livers of lean and obese mice. METHODS: Lean and ob/ob male mice (5-week-old) were treated for 4 months with a mixture of 11 drugs (acetaminophen, caffeine, carbamazepine, cotinine, diclofenac, erythromycin, ibuprofen, phenazone, roxithromycin, salicylic acid and sulfamethoxazole) provided in drinking water at a concentration of 1 mg/L (for each drug). At the end of the treatment, investigations were performed in liver and plasma. RESULTS: Some liver and plasma abnormalities were observed in ob/ob mice treated with the cocktail containing 1 mg/L of each drug. For this dosage, a gene expression analysis by microarray showed altered expression of circadian genes (e.g. Bmal1, Dbp, Cry1) in lean and obese mice. RT-qPCR analyses carried out in all groups of animals indicated that expression of 8 different circadian genes was significantly modified in a dose-dependent manner. For some genes, a significant modification was observed for dosages as low as 100-1,000 ng/L. Drug mixture and obesity presented an additive effect on circadian gene expression. These data were confirmed in an independent study performed in female mice. CONCLUSIONS: Chronic, low dose exposure to pharmaceuticals disturbed hepatic expression of circadian genes, especially in obese mice. Because some of the 11 drugs can be found in the drinking water at such concentrations (e.g. acetaminophen, carbamazepine, ibuprofen) our data could be relevant in environmental toxicology, in particular for obese individuals exposed to these contaminants. C57BL/6J lean and ob/ob male mice (5-week-old) were treated for 4 months with a mixture of 11 drugs provided in drinking water at a concentration of 1 mg/L (for each drug). 4 groups were designed: untreated versus treated WT and ob/ob mice (n=6 mice per group).

Dataset Information

Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: a Proteomics study

Publications

Stress response of lettuce (Lactuca sativa) to environmental contamination with selected pharmaceuticals: A proteomic study.

Similar Datasets

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