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High throughput microfluidic 3D biomimetic model enabling quantitative description of human breast tumor microenvironment

ABSTRACT: Cancer is driven by genetic aberrations in the tumor cells and fundamental changes in the tumor microenvironment (TME). These changes offer potential targets for novel therapeutics, yet lack of in vitro 3D models recapitulating this complex microenvironment impedes such progress. Here, we generated tumor-stroma spheroids reflecting the in vivo TME of early and advanced breast tumor, using a high throughput microfluidic system. The spheroids, composed of tumor cells, fibroblasts and activated immune cells, were generated and cultivated on-chip in alginate (Alg) or alginate-alginate sulfate (Alg/Alg-S) hydrogels.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Michal Gregus

LAB HEAD: Alexander R. Ivanov

PROVIDER: PXD021509 | Pride | 2022-10-13

REPOSITORIES: Pride

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High-throughput microfluidic 3D biomimetic model enabling quantitative description of the human breast tumor microenvironment.

Berger Fridman Ilana I Kostas James J Gregus Michal M Ray Somak S Sullivan Matthew R MR Ivanov Alexander R AR Cohen Smadar S Konry Tania T

Acta biomaterialia 20210618

Cancer is driven by both genetic aberrations in the tumor cells and fundamental changes in the tumor microenvironment (TME). These changes offer potential targets for novel therapeutics, yet lack of in vitro 3D models recapitulating this complex microenvironment impedes such progress. Here, we generated several tumor-stroma scaffolds reflecting the dynamic in vivo breast TME, using a high throughput microfluidic system. Alginate (Alg) or alginate-alginate sulfate (Alg/Alg-S) hydrogels were used ...[more]

PMID: 34153511

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