Dataset Information

Biostimulant impacts of Glutacetine® and derivated formulations on bread wheat grain proteome

ABSTRACT: Nitrogen (N) fertilization is essential in order to insure wheat bread yield and quality. Improving nitrogen use efficiency is therefore crucial for wheat grain protein quality. In the present work, we analysed the effects of new biostimulants containing Glutacetine® or derivate formulations that have been mixed with urea-ammonium-nitrate fertilizer (UAN) on winter wheat grain proteome. A largescale quantitative proteomics analysis of 2 wheat flour fractions led to a dataset of 4369 identified proteins. Quantitative analysis revealed 9, 39 and 96 proteins with a significantly varying abundance after Glutacetine®, VNT1 and VNT4 treatment, respectively, with 11 proteins (or homologue) which were affected by 2 different biostimulants. Major effects affected proteins involved in regulation processes with transcription regulator proteins, in stress responses with biotic and abiotic stress defence proteins, in flowering efficiency with proteins involved in pollen development, in storage functions with the gluten protein alpha-gliadins and starch synthase and in seed development with proteins implied in transport, proteases activity, energy machinery and N pathway. Altogether, our controlled conditions study showed that Glutacetine®, VNT1 and VNT4 biostimulants positively affected protein composition for grain quality.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Seed

SUBMITTER: VICTOR MAIGNAN

LAB HEAD: Jean-Christophe AVICE

PROVIDER: PXD021513 | Pride | 2021-05-21

REPOSITORIES: Pride

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Publications

Biostimulant impacts of Glutacetine® and derived formulations (VNT1 and VNT4) on the bread wheat grain proteome.

Maignan Victor V Bernay Benoit B Géliot Patrick P Avice Jean-Christophe JC

Journal of proteomics 20210513

Nitrogen (N) fertilizer is essential to ensure grain yield and quality in bread wheat. Improving N use efficiency is therefore crucial for wheat grain protein quality. In the present work, we analysed the effects on the winter wheat grain proteome of biostimulants containing Glutacetine® or two derived formulations (VNT1 and 4) when mixed with urea-ammonium-nitrate fertilizer. A large-scale quantitative proteomics analysis of two wheat flour fractions produced a dataset of 4369 identified protei ...[more]

PMID: 33992839

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Project description:Durum wheat requires high nitrogen inputs to obtain the high protein concentration necessary to satisfy pasta and semolina quality criteria. Optimizing plant nitrogen use efficiency is therefore of major importance for wheat grain quality. Here, we studied the impact on grain yield, protein concentration, and for the first time on protein composition of a marine (DPI4913) and a fungal (AF086) biostimulants applied to plant leaves. Plants were grown in a greenhouse and sampled at maturity for grain analysis. The protein concentration and quantity in grains per plant were determined, as well as water-use efficiency. A large-scale quantitative proteomics study of wheat flour samples was performed to determine the effect of biostimulant treatment on grain protein composition. Grain dry biomass and protein quantity were increased by 23.9% and 24.8% respectively with DPI4913, and by 27.4% and 25.9%, respectively, with AF086. A total of 1471 proteins were identified and 1391 were quantified. Quantitative proteomics analysis revealed 26 and 38 proteins with a significantly varying abundance after DPI4913 and AF086 treatment, respectively. Among these, 14 proteins were affected by both DPI4913 and AF086 treatments. The highest variations in proteins abundances were found for proteins involved in grain technological properties such as grain hardness, in storage functions with the substantial over-representation of the gluten protein gamma-gliadin, in regulation processes with the over-representation of proteins that play a transcription regulator role, and in stress responses with the over-representation of proteins implied in biotic and abiotic stress defense. The involvement of biostimulants in the abiotic stress response is suggested by the increase in water-use efficiency for DPI4913 treatment (15.4%) and for AF086 treatment (9.9%). In conclusion, our work, performed in greenhouse, has shown that DPI4913 and AF086 treatments promote grain yield while maintaining protein concentration in grains, and positively affect protein composition in term of grain quality. This study suggests that these biostimulants could be used to optimize durum wheat production and quality in field conditions.

Project description:Purpose: To identify abiotic stress responsive and tissue specific miRNAs at genome wide level in wheat (Triticum aestivum) Results: Small RNA libraries were constructed from four tissues (root, shoot, mature leaf and spikelets) and three stress treatments of wheat seedlings (control, high temperature, salinity and water-deficit). A total of 59.5 million reads were obtained by high throughput sequencing of eight wheat libraries, of which 32.5 million reads were found to be unique. Using UEA sRNA workbench we identified 47 conserved miRNAs belonging to 20 families, 1030 candidate novel and 51 true novel miRNAs. Several of these miRNAs displayed tissue specific expression whereas few were found to be responsive to abiotic stress treatments. Target genes were predicted for miRNAs identified in this study and their grouping into functional categories revealed that the putative targets were involved in diverse biological processes. RLM-RACE of predicted targets of three conserved miRNAs (miR156, miR160 and miR164) confirmed their mRNA cleavage, thus indicating their regulation at post-transcriptional level by corresponding miRNAs. Expression profiling of confirmed target genes of these miRNAs was also performed. Conclusions: This is the first comprehensive study on profiling of miRNAs in a variety of tissues and in response to several abiotic stresses in wheat. Our findings provide valuable resource for better understanding on the role of miRNAs in stress tolerance as well as plant development. Additionally, this information could be utilized for designing wheat plants for enhanced abiotic stress tolerance and higher productivity. Total eight (three stress, one control and four tissue specific small RNA libraries were pepared and sequenced independently [wheat control (WC), wheat high temperature stressed (WHTS), wheat salinity stressed (WSS) and wheat drought stressed (WDS), wheat shoot(WSH), wheat leaf (WLF), wheat flower(WFL), wheat root(WRT)] on Illumina GAIIx

Dataset Information

Biostimulant impacts of Glutacetine® and derivated formulations on bread wheat grain proteome

Publications

Biostimulant impacts of Glutacetine® and derived formulations (VNT1 and VNT4) on the bread wheat grain proteome.

Similar Datasets

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