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Identification of RPA1 interactome in mouse liver tissue

ABSTRACT: To examine the molecular mechanisms by which RPA1 regulates transcription in liver, we conducted immunoprecipitation followed by mass spectrometry to characterize RPA1-associated proteins in mouse liver.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Qi Yin

LAB HEAD: Yuxin Yin

PROVIDER: PXD021529 | Pride | 2022-07-19

REPOSITORIES: Pride

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RPA1 controls chromatin architecture and maintains lipid metabolic homeostasis.

Yin Qi Q Li Yang Y Zhou Zhe Z Li Xiang X Li Minghao M Liu Chengyang C Dong Di D Wang Guangxi G Zhu Minglu M Yang Jingyi J Jin Yan Y Guo Limei L Yin Yuxin Y

Cell reports 20220701 2

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease, with a prevalence of 25% worldwide. However, the underlying molecular mechanism involved in the development and progression of the NAFLD spectrum remains unclear. Single-stranded DNA-binding protein replication protein A1 (RPA1) participates in DNA replication, recombination, and damage repair. Here, we show that Rpa1<sup>+/-</sup> mice develop fatty liver disease during aging and in response to a high-fat diet. Liver-sp ...[more]

PMID: 35830798

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