Human cancer cell MCF-7 IP-PTEN MS

ABSTRACT: The MCF-7 cells were lysed using RIPA lysis buffer with a high salinity content to break out non-covalent binding of proteins. Then IP under partially denaturing conditions was performed and demonstrated that anti-PTEN precipitates were detected as smear bands, then mass spectrometry analysis of the smear bands was performed.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Zhiqiang Peng  

LAB HEAD: Ping Xie

PROVIDER: PXD021544 | Pride | 2022-04-04

REPOSITORIES: Pride