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Human cancer cell MCF-7 IP-PTEN MS

ABSTRACT: The MCF-7 cells were lysed using RIPA lysis buffer with a high salinity content to break out non-covalent binding of proteins. Then IP under partially denaturing conditions was performed and demonstrated that anti-PTEN precipitates were detected as smear bands, then mass spectrometry analysis of the smear bands was performed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Zhiqiang Peng

LAB HEAD: Ping Xie

PROVIDER: PXD021544 | Pride | 2022-04-04

REPOSITORIES: Pride

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Neddylation of PTEN regulates its nuclear import and promotes tumor development.

Xie Ping P Peng Zhiqiang Z Chen Yujiao Y Li Hongchang H Du Mengge M Tan Yawen Y Zhang Xin X Lu Zhe Z Cui Chun-Ping CP Liu Cui Hua CH He Fuchu F Zhang Lingqiang L

Cell research 20201209 3

PTEN tumor suppressor opposes the PI3K/Akt signaling pathway in the cytoplasm and maintains chromosomal integrity in the nucleus. Nucleus-cytoplasm shuttling of PTEN is regulated by ubiquitylation, SUMOylation and phosphorylation, and nuclear PTEN has been proposed to exhibit tumor-suppressive functions. Here we show that PTEN is conjugated by Nedd8 under high glucose conditions, which induces PTEN nuclear import without effects on PTEN stability. PTEN neddylation is promoted by the XIAP ligase ...[more]

PMID: 33299139

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