Protein turnover rates within mitochondrial complexes in mitochondrial disease patients and in a DNAJC30 knock-out cell line

ABSTRACT: We identified DNAJC30 as a key protein in the maintenance of functional NADH:ubiquinone oxidoreductase (complex I). To determine the turnover rates of modules in complex I and other mitochondrial complexes, we pulsed both patient and control fibroblast cell lines and a DNAJC30 knock-out HEK and control HEK cell line over 12 hours with heavy amino acids (PulseSILAC) followed by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Ilka Wittig  

LAB HEAD: Holger Prokisch

PROVIDER: PXD021548 | Pride | 2021-09-09

REPOSITORIES: Pride