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Venom profiling of the insular species Bothrops alcatraz: characterization of proteome, glycoproteome, and N-terminome using Terminal Amine Isotopic Labeling of Substrates.

ABSTRACT: In this study we applied mass spectrometric approaches to characterize the proteome and glycoproteome of B. alcatraz venom, and to show for the first time the use of the TAILS method to profile a snake venom N-terminome.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bothrops Alcatraz

SUBMITTER: Debora Andrade Silva

LAB HEAD: Solange Maria de Toledo Serrano

PROVIDER: PXD021561 | Pride | 2021-01-13

REPOSITORIES: Pride

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Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.

Andrade-Silva Débora D Zelanis André A Travaglia-Cardoso Silvia R SR Nishiyama Milton Y MY Serrano Solange M T SMT

Journal of proteome research 20210106 2

Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat. We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz). Here, we characterized B. alcatraz venom using electrophoretic, proteom ...[more]

PMID: 33404253

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