Ontology highlight
ABSTRACT:
INSTRUMENT(S): Synapt MS
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Petr Man
LAB HEAD: Petr Man
PROVIDER: PXD021591 | Pride | 2022-02-15
REPOSITORIES: Pride
Bar-El Michal Lisnyansky ML Vaňková Pavla P Yeheskel Adva A Simhaev Luba L Engel Hamutal H Man Petr P Haitin Yoni Y Giladi Moshe M
Nature communications 20201019 1
The human cis-prenyltransferase (hcis-PT) is an enzymatic complex essential for protein N-glycosylation. Synthesizing the precursor of the glycosyl carrier dolichol-phosphate, mutations in hcis-PT cause severe human diseases. Here, we reveal that hcis-PT exhibits a heterotetrameric assembly in solution, consisting of two catalytic dehydrodolichyl diphosphate synthase (DHDDS) and inactive Nogo-B receptor (NgBR) heterodimers. Importantly, the 2.3 Å crystal structure reveals that the tetramer assem ...[more]