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Thiol-ene microfluidic chip for fast on-chip sample clean-up, separation and ESI mass analysis of peptides and proteins

ABSTRACT: Microfluidic devices coupled to MS promises low manufacturing costs, low sample consumption and channels with a high surface area to volume ratio and tailorable functional groups. Here, we describe a thiol-ene-based microfluidic chip that is capable of sample loading, desalting and on-chip reversed phase chromatography prior to on-chip electrospray ionization for the intact analysis of proteins and peptides, as well as, for on-chip bottom-up proteomics workflows coupled directly with mass spectrometry.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Rasmus Svejdal

LAB HEAD: Kasper D. Rand

PROVIDER: PXD021658 | Pride | 2020-10-19

REPOSITORIES: Pride

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