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Protein complexes involving haemolysin BL of Bacillus cereus

ABSTRACT: Bacillus cereus Hemolysin BL is a tripartite toxin responsible for a diarrheal type of food poisoning.We found distinct complexes of up to 600 kDa on native gels, whose structure and size were primarily defined by Hbl B. Hbl L1 and L2 were also identified in these complexes using Western blotting and an LC-MS approach. LC-MS also revealed that many other proteins secreted by B. cereus exist in complexes.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bacteria Bacillus Cereus F837/76

SUBMITTER: Nadja Jessberger

LAB HEAD: Nadja Jessberger

PROVIDER: PXD021720 | Pride | 2020-10-30

REPOSITORIES: Pride

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Characteristics of the Protein Complexes and Pores Formed by <i>Bacillus cereus</i> Hemolysin BL.

Jessberger Nadja N Dietrich Richard R Schauer Kristina K Schwemmer Stefanie S Märtlbauer Erwin E Benz Roland R

Toxins 20201024 11

<i>Bacillus cereus</i> Hemolysin BL is a tripartite toxin responsible for a diarrheal type of food poisoning. Open questions remain regarding its mode of action, including the extent to which complex formation prior to cell binding contributes to pore-forming activity, how these complexes are composed, and the properties of the pores formed in the target cell membrane. Distinct complexes of up to 600 kDa were found on native gels, whose structure and size were primarily defined by Hbl B. Hbl L1 ...[more]

PMID: 33114414

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