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Proteome content of insoluble fraction of Httex1 transfected cells

ABSTRACT: Investigate the protein content of the insoluble fraction of HEK cells transfected with Httex1 (+/-GFP).The ultimate goal is to assess the protein contents of Httex1 transfected cells depending on the polyQ length and the presence of a GFP tag.

INSTRUMENT(S): Orbitrap Fusion Lumos, MaxQuant

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293 Cell

DISEASE(S): Huntington Disease

SUBMITTER: Nathan Riguet

LAB HEAD: Hilal A. Lashuel

PROVIDER: PXD021742 | Pride | 2021-10-08

REPOSITORIES: Pride

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Nuclear and cytoplasmic huntingtin inclusions exhibit distinct biochemical composition, interactome and ultrastructural properties.

Riguet Nathan N Mahul-Mellier Anne-Laure AL Maharjan Niran N Burtscher Johannes J Croisier Marie M Knott Graham G Hastings Janna J Patin Alice A Reiterer Veronika V Farhan Hesso H Nasarov Sergey S Lashuel Hilal A HA

Nature communications 20211112 1

Despite the strong evidence linking the aggregation of the Huntingtin protein (Htt) to the pathogenesis of Huntington's disease (HD), the mechanisms underlying Htt aggregation and neurodegeneration remain poorly understood. Herein, we investigated the ultrastructural properties and protein composition of Htt cytoplasmic and nuclear inclusions in mammalian cells and primary neurons overexpressing mutant exon1 of the Htt protein. Our findings provide unique insight into the ultrastructural propert ...[more]

PMID: 34772920

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Proteome content of insoluble fraction of Httex1 transduced neurons

Project description:Investigate the protein content of the insoluble fraction of primary neurons transdusced with Httex1 (+/-GFP).The ultimate goal is to assess the protein contents of Httex1 transduced neurons depending on the polyQ length and the presence of a GFP tag.

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Differentially expressed miRNAs in TGF-M-NM-21-knock down human colorectal cancer cell lines vs controls

Project description:To investigate the role of TGF-M-NM-21-regulated miRNAs in the progression of colorectal cancer,we performed comprehensive miRMA microarray analysis on RNA derived from typical human colorectal cancer cell lines and TGF-M-NM-21 knock-down human colorectal cancer cell lines. We identified a novel set of TGF-M-NM-21-related miRNAs. Total RNA was isolated from TGF-M-NM-21-knock down colorecatl cancer cell lines and controls.Three-condition experiment: shRNA-TGF-M-NM-21/Lovo cells vs. shRNA-Control/Lovo cells, shRNA-TGF-M-NM-21/SW620 cells vs. shRNA-Control/ SW620 cells, and shRNA-TGF-M-NM-21/HT29 cells vs. shRNA-Control/HT29 cells. Biological replicates: 1 Lovo cells stably transfected with shRNA-TGF-M-NM-21- pSUPER gfp-neo, 1SW620 cells stably transfected with shRNA-TGF-M-NM-21- pSUPER gfp-neo, 1HT29 cells stably transfected with shRNA-TGF-M-NM-21- pSUPER gfp-neo, 1Love cells stably transfected with shRNA-Control- pSUPER gfp-neo, 1SW620 cells stably transfected with shRNA-Control- pSUPER gfp-neo, and 1HT29 cells stably transfected with shRNA-Control- pSUPER gfp-neo, independently grown and harvested. One replicate per array.

2013-12-16 | E-GEOD-53338 | biostudies-arrayexpress

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