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Identification of proteins in the interactomes of 4 DNA-repair proteins of Deinococcus radiodurans

ABSTRACT: Project includes identification of the interacting partners of the four important DNA-repair proteins (DdrA, DdrB, RecA, Ssb) of the extremophile D. radiodurans. The interacting proteins were crosslinked with DSP, immunoprecipitated using antibodies against the four DNA repair proteins, followed by protein identification by LC MS/MS.

INSTRUMENT(S): 6540 Q-TOF LC/MS

ORGANISM(S): Deinococcus Radiodurans R1

SUBMITTER: Mahesh Kumar Padwal

LAB HEAD: Dr. BHAKTI BASU

PROVIDER: PXD021822 | Pride | 2021-05-07

REPOSITORIES: Pride

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An in vivo Interaction Network of DNA-Repair Proteins: A Snapshot at Double Strand Break Repair in Deinococcus radiodurans.

Ujaoney Aman Kumar AK Padwal Mahesh Kumar MK Basu Bhakti B

Journal of proteome research 20210430 6

An extremophile Deinococcus radiodurans survives massive DNA damage by efficiently mending hundreds of double strand breaks through homology-dependent DNA repair pathways. Although DNA repair proteins that contribute to its impressive DNA repair capacity are fairly known, interactions among them or with proteins related to other relevant pathways remain unexplored. Here, we report in vivo cross-linking of the interactomes of key DNA repair proteins DdrA, DdrB, RecA, and Ssb (baits) ...[more]

PMID: 33929844

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