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Impact of KsgA/Dim1 on proteostasis in Haloferax volcanii and Escherichia coli

ABSTRACT: Shotgun label-free analysis of Haloferax volcanii wildtype cells in comparison to strains deleted and catalytically impaired of the almost universally conserved ribosomal RNA dimethyltransferase KsgA/Dim1and of Escherichia coli wildtype strain in comparison to KsgA/Dim1 deleted strain.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Escherichia Coli Haloferax Volcanii (halobacterium Volcanii)

SUBMITTER: Marlene Oeffinger

LAB HEAD: Marlene Heloise Oeffinger

PROVIDER: PXD021827 | Pride | 2021-01-25

REPOSITORIES: Pride

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Insights into synthesis and function of KsgA/Dim1-dependent rRNA modifications in archaea.

Knüppel Robert R Trahan Christian C Kern Michael M Wagner Alexander A Grünberger Felix F Hausner Winfried W Quax Tessa E F TEF Albers Sonja-Verena SV Oeffinger Marlene M Ferreira-Cerca Sébastien S

Nucleic acids research 20210201 3

Ribosomes are intricate molecular machines ensuring proper protein synthesis in every cell. Ribosome biogenesis is a complex process which has been intensively analyzed in bacteria and eukaryotes. In contrast, our understanding of the in vivo archaeal ribosome biogenesis pathway remains less characterized. Here, we have analyzed the in vivo role of the almost universally conserved ribosomal RNA dimethyltransferase KsgA/Dim1 homolog in archaea. Our study reveals that KsgA/Dim1-dependent 16S rRNA ...[more]

PMID: 33434266

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