Glycoproteomic analysis of Haloferax volcanii strains H53, DaglB, and Dagl15

ABSTRACT: An in-depth glycoproteomic analysis of Haloferax volcanii has been performed, comparing the wildtype H53 with knockout mutants of aglB and agl15. While AglB-dependent glycosylation has been described to occur under standard culture conditions, the Agl15-dependent glycosylation pathway has previously been described to be only active under low-salt conditions. In this dataset, the largest archaeal glycoproteome could be identified with more than 40 N-glycoproteins and more than 100 N-glycopptides. It reveals not only that both glycosylation pathways can be active under the same conditions but also that the same glycosites can be modified by different glycans from both glycosylation pathways.

INSTRUMENT(S):

ORGANISM(S): Haloferax Volcanii (halobacterium Volcanii)

SUBMITTER: Stefan Schulze  

LAB HEAD: Mechthild Pohlschroder

PROVIDER: PXD021874 | Pride | 2021-06-20

REPOSITORIES: Pride