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Xylem sap shotgun proteomics from Mn-excess tomato plants

ABSTRACT: The aim of this work was to study the effects of Mn excess on the protein profile of the xylem sap of tomato (Solanum lycopersicum), with the aim of elucidating plant response mechanisms to these nutritional stresses. Tomato was chosen as a model plant because the tomato genome has been published and this plant species has adequate root pressure and turgid stems that permit xylem sap sampling in sufficient amounts by de-topping. The high- throughput shotgun analysis has permitted to identify and quantitate a large number of low abundance proteins in the tomato xylem sap.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Xylem

SUBMITTER: Laura Ceballos-Laita

LAB HEAD: Javier Abadia

PROVIDER: PXD021973 | Pride | 2020-12-10

REPOSITORIES: Pride

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Effects of Excess Manganese on the Xylem Sap Protein Profile of Tomato (Solanum lycopersicum) as Revealed by Shotgun Proteomic Analysis.

Ceballos-Laita Laura L Gutierrez-Carbonell Elain E Takahashi Daisuke D Lonsdale Andrew A Abadía Anunciación A Doblin Monika S MS Bacic Antony A Uemura Matsuo M Abadía Javier J López-Millán Ana Flor AF

International journal of molecular sciences 20201123 22

Metal toxicity is a common problem in crop species worldwide. Some metals are naturally toxic, whereas others such as manganese (Mn) are essential micro-nutrients for plant growth but can become toxic when in excess. Changes in the composition of the xylem sap, which is the main pathway for ion transport within the plant, is therefore vital to understanding the plant's response(s) to metal toxicity. In this study we have assessed the effects of exposure of tomato roots to excess Mn on the protei ...[more]

PMID: 33238539

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